Percoll-purified lung leukocytes from collagenase digested lung tissue or magnetically isolated CD4+ T-cells (3x106 per sample) were cultured for 24 h with or without additional skewing cytokines. Clarified supernatants were subsequently analyzed for the indicated cytokines using Duo-set ELISA assays (R & D systems, Minneapolis, MN). Recombinant murine DLL4 (R&D systems, Minneapolis, MN) was plate bound to tissue culture dishes at a concentration of 50 nM in PBS for 3 h at 37°C. Plates were washed 3 X with sterile PBS before addition of purified leukocytes in complete media.
Recombinant murine dll4
Manufactured by R&D Systems
Recombinant murine DLL4 is a protein produced in a mammalian cell expression system. It is a member of the Delta-like protein family and plays a role in the Notch signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant murine dll4
1
Ex Vivo Skewing of Lung Leukocytes
2
Ex Vivo Skewing of Lung Leukocytes
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Percoll-purified lung leukocytes from collagenase digested lung tissue or magnetically isolated CD4+ T-cells (3x106 per sample) were cultured for 24 h with or without additional skewing cytokines. Clarified supernatants were subsequently analyzed for the indicated cytokines using Duo-set ELISA assays (R & D systems, Minneapolis, MN). Recombinant murine DLL4 (R&D systems, Minneapolis, MN) was plate bound to tissue culture dishes at a concentration of 50 nM in PBS for 3 h at 37°C. Plates were washed 3 X with sterile PBS before addition of purified leukocytes in complete media.
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