Blood from healthy human donors was extracted from peripheral veins and collected in heparin-coated syringes (Informed consent was obtained from the donors and the procedure was approved by the local ethics committee). PMN were isolated by centrifugation on PolymorphPrep (Axis-Shield PoC AS, Oslo, Norway) according to the protocol provided by the manufacturer. Isolated PMN were suspended in McCoys medium (Biochrom) and used within one hour. PMN lysate was prepared by the nitrogen cavitation method. PMN in a concentration of 1x108 were suspended in 1ml phosphate buffer and then cavitated in nitrogen for 20 min at 380 psi.
Mccoys medium
Manufactured by Harvard Bioscience
Sourced in Germany
McCoys medium is a commonly used cell culture medium for the growth and maintenance of various cell types, including human and animal cell lines. It provides the necessary nutrients, vitamins, and minerals to support the in vitro cultivation of cells.
Automatically generated - may contain errors
Lab products found in correlation
Polymorphprep, by Axis-Shield (1 mentions)
Gentamycin, by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Ovcar 3 cells, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 protocols using mccoys medium
1
Isolation and Lysis of Human Neutrophils
2
Endometrial Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
Endometrial cancer cells were obtained from ATCC (Manassas, VA, USA). HEC-1-A were cultivated in McCoys medium (Biochrom AG, Berlin, Germany). KLE and RL 95-2 were cultivated in DMEM/F12 medium (Invitrogen, Karlsruhe, Germany) and AN3-CA ware cultivated in MEM Earleʼs medium (Invitrogen). All media contained 10% fetal bovine serum and 50 µg/mL gentamycin (Invitrogen) and cells were cultivated at 37 °C in a 5% CO
2humidified atmosphere.
Cell lines differ in their grading and pattern of metastatic spread; the cells of HEC-1 A and RL 95-2 are moderately differentiated and originate from the epithelial layer of the endometrium. KLE and AN3-CA are poorly differentiated cell lines of endometrial adenocarcinoma, with AN3-CA originating from lymph node metastasis
15 (link)
.
Cell lines were cultured 48 h prior to the different experiments. Different numbers of cells were used for all experiments as follows: 50 000 RL 95-2 cells, 30 000 HEC-1A cells, 15 000 KLE cells and 40 000 AN3CA cells.
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Endometrial cancer cells were obtained from ATCC (Manassas, VA, USA). HEC-1-A were cultivated in McCoys medium (Biochrom AG, Berlin, Germany). KLE and RL 95-2 were cultivated in DMEM/F12 medium (Invitrogen, Karlsruhe, Germany) and AN3-CA ware cultivated in MEM Earleʼs medium (Invitrogen). All media contained 10% fetal bovine serum and 50 µg/mL gentamycin (Invitrogen) and cells were cultivated at 37 °C in a 5% CO
2humidified atmosphere.
Cell lines differ in their grading and pattern of metastatic spread; the cells of HEC-1 A and RL 95-2 are moderately differentiated and originate from the epithelial layer of the endometrium. KLE and AN3-CA are poorly differentiated cell lines of endometrial adenocarcinoma, with AN3-CA originating from lymph node metastasis
15 (link)
.
Cell lines were cultured 48 h prior to the different experiments. Different numbers of cells were used for all experiments as follows: 50 000 RL 95-2 cells, 30 000 HEC-1A cells, 15 000 KLE cells and 40 000 AN3CA cells.
3
Cultivation of SKOV-3 and OVCAR-3 Cell Lines
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SKOV-3 cells were cultivated in McCoys medium (Biochrom AG, Berlin, Germany) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Invitrogen, Karlsruhe, Germany) at 37°C in a 5% CO2 humidified atmosphere. OVCAR-3 cells purchased from ATCC (Manassas, Virginia, USA) were cultivated in RPMI medium (Invitrogen) containing 10% fetal bovine serum, 1% penicillin-streptomycin and 0.01 mg/ml insulin under conditions described above.
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