Anti mouse igg1 hrp

Manufactured by Abcam

Sourced in United Kingdom

Anti-mouse IgG1-HRP is a secondary antibody that binds to the IgG1 subclass of mouse immunoglobulins and is conjugated to horseradish peroxidase (HRP). It can be used for detection and quantification of mouse IgG1 in various immunoassays.

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Lab products found in correlation

3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions) Elisa, by Corning (1 mentions) Nunc maxisorp 96 well plate, by Thermo Fisher Scientific (1 mentions) 1 step ultra tmb, by Thermo Fisher Scientific (1 mentions) Phosphorylated iκb α, by Abcam (1 mentions) Phosphorylated stat3, by Abcam (1 mentions) Cathepsin k, by Abcam (1 mentions) Mmp 9, by Abcam (1 mentions) Mcp 1, by Abcam (1 mentions) Complete freund s adjuvant, by Merck Group (1 mentions)

3 protocols using anti mouse igg1 hrp

ELISA Assay for Antibody Detection

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The 8-well strip was coated with the CU44 or Gy684 HA proteins in a total volume of 100 µl (2 ng/µl) for ELISA (Corning) overnight at 4 °C. Following 1% of BSA blocking and PBS washing, primary antibody from diluted serum samples were bound for 1 h at 37 °C, washed with PBS and detected by anti-mouse IgG1-HRP (Abcam, Cambridge, UK) as the secondary antibody for 1 h at 37 °C. 150 µl of 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich) was used as substrate and the reaction was stopped by addition of 2 N sulfuric acid, and absorbance at 450 nm was monitored.

Seok J.H., Kim J., Lee D.B., Cho K.J., Lee J.H., Bae G., Chung M.S, & Kim K.H. (2017). Conformational modulation of influenza virus hemagglutinin: characterization and in vivo efficacy of monomeric form. Scientific Reports, 7, 7540.

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Norovirus VLP Quantification Assay

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Mouse monoclonal IgG2 anti-norovirus antibodies (Maine Biotech, MAB228 (GI); MAB227 (GII)) diluted 1:2000 in PBS were coated on Nunc MaxiSorp 96-well plates (Fisher Scientific, Pittsburgh, PA) overnight at 4°C. The wells were washed 5 times with wash buffer, and then blocked for 1 h at room temperature in blocking buffer. Norovirus VLPs at indicated concentrations were diluted in blocking buffer, and allowed to incubate on the plate at room temperature for 1 h. The wells were washed 3 times with wash buffer, followed by incubation with corresponding mouse monoclonal IgG1 anti-norovirus antibodies (Millipore, MAB80143 (GI); Maine Biotech MAB226 (GII)) diluted 1:2000 in blocking buffer for 1 h at room temperature. The wells were washed 3 times with wash buffer, followed by incubation with a polyclonal anti-mouse IgG1:HRP (Abcam, Cambridge, MA) diluted 1:2000 in blocking buffer for 1 h at room temperature. Finally, the wells were washed 3 times with wash buffer and were developed using 1-step Ultra TMB according to manufacturer’s protocol (Thermo Scientific, Waltham, MA). The OD at 450 nm was measured and plotted against known VLP concentrations.

Springer M.J., Ni Y., Finger-Baker I., Ball J.P., Hahn J., DiMarco A.V., Kobs D., Horne B., Talton J.D, & Cobb R.R. (2016). Preclinical Dose Ranging Studies of a Novel Dry Powder Norovirus Vaccine Formulation. Vaccine, 34(12), 1452-1458.

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Flemingia philippinensis Anti-Inflammatory Assay

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The Flemingia philippinensis root was purchased from a traditional herb market in Guilin, China, and authenticated by a taxonomist. The voucher specimen was stored in Pharmacy College, Guilin Medical University, Guilin, China. Chicken type collagen (CII) and Complete Freund's Adjuvant were purchased from Sigma-Aldrich. Antibodies to the following proteins were purchased from Santa Cruz Biotechnology, Santa Cruz, CA: goat anti-mouse IgG2a-HRP (sc-2061), MMP-9 (sc-393859), cathepsin K (sc-48353), MCP-1 (sc-52701), phosphorylated STAT3 (sc-8059), phosphorylated I-κBα (sc-8404), and phosphorylated NF-κBp65 (sc-101753); antibodies anti-Mouse IgG1-HRP, phosphorylated JNK1+JNK2+JNK3 (ab124956), phosphorylated p38 MAPK (ab178867), and phosphorylated Jun D+c-Jun (ab208035) were purchased from Abcam; phosphorylated ERK1/2 antibody (4370) was from Cell Signaling Technology, GAPDH antibody was from PeproTech Biotechnology, and ELISA kits for TNF-α, IL-10, IL-12/IL-23 p40 (total), and MCP-1 were from eBioscience.

Sun G., Xing C., Zeng L., Huang Y., Sun X, & Liu Y. (2019). Flemingia philippinensis Flavonoids Relieve Bone Erosion and Inflammatory Mediators in CIA Mice by Downregulating NF-κB and MAPK Pathways. Mediators of Inflammation, 2019, 5790291.

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