Blood was collected using BD Vacutainer plastic serum tubes (BD Biosciences, Heidelberg, Germany). Serum was prepared from clotted blood samples by centrifugation for 10 min at 1,700×g, RT and stored in aliquots at −80°C. Serum used for ADCC assays was heat inactivated at 56° for 30 min. Serum as a source for complements in CDC assays was used without further manipulation.
Vacutainer plastic serum tube
Manufactured by BD
Sourced in United States
BD Vacutainer plastic serum tubes are used for the collection and transportation of blood samples. They are designed to separate the serum component from the blood cells for clinical analysis.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using vacutainer plastic serum tube
1
Serum Preparation for ADCC and CDC Assays
2
Blood Sample Collection and Storage
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Blood was drawn from the participants between 9:00 and 15:00 and at three centres, using the same study protocol and BD Vacutainer plastic serum tubes (BD, New Jersey, USA). The tubes were processed within 1 hour by centrifugation at 1800 ×g for 10 minutes and subsequently stored in polypropylene tubes (Sarstedt, Germany) at −80°C.
3
Peripheral Blood and Aqueous Humor Sampling
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Peripheral blood (ear vein) was collected 15 minutes and 4 hours after EdU injection and kept in a blood collection tube (BD Vacutainer plastic serum tube; BD, Franklin Lakes, NJ) at 5 °C for 30 minutes, before centrifugation at 2500 rpm at room temperature (RT) for 15 minutes. AH samples (approximately 300 µL) were collected through the limbus with a 27G needle after 15 minutes (right eye) and after 4 hours (left eye), and the animals were euthanized with intravenous pentobarbital. The samples were kept in Eppendorf tubes at −80 °C until further processing.
4
Biomarker Sampling in Cardiac Perfusion
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Perfusate samples were collected during COMP and normothermic testing in Lithium-Heparin (BD Vacutainer plastic Lithium-Heparin tube) and Serum tubes (BD vacutainer plastic serum tube), centrifuged at 2300G (2 × 10 minutes), aliquoted and stored at −80°C. Samples were collected during hypothermic perfusion (5, 90, and 240 minutes) and normothermic perfusion (baseline, 30, 60, 90, 120, 180, and 240 minutes). As weight could impact the results, variables analyzed were normalized to the mean weight of 450 g of all hearts included (variable was divided by the weight of the heart and multiplied by the mean weight of 450 g).
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