Recombinant human PDGF-BB, trypan blue reagent, the phosphoinositide 3-kinase (PI3K) specific inhibitor, LY294002, the GSK-3β antagonist, SB415286, and cell proliferation reagent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma, St. Louis, MO, USA. The proliferating cell nuclear antigen (PCNA) antibody was purchased from Cell Signaling Technology (Product no. 2586s). Trypsin-ethylenediaminetetraacetic acid (EDTA) (0.25%), Dulbecco's modified Eagle's medium/F12 (DMEM/F12) and fetal bovine serum (FBS) were from PromoCell (Heidelberg, Germany). The digoxin injection was acquired from Minsheng Pharmaceutical Group Co., Ltd. (Hangzhou, China). Digoxin was purchased from J&K Scientific Ltd. (Beijing, China) and dissolved in dimethyl sulfoxide (DMSO), and the concentration of DMSO was <0.8% in the control and drug-containing medium.
Proliferating cell nuclear antigen antibody
Manufactured by Cell Signaling Technology
Sourced in United States
Proliferating cell nuclear antigen (PCNA) antibody is a lab equipment product that detects the PCNA protein, which is essential for DNA replication and cell cycle progression. The antibody can be used to identify and quantify PCNA expression in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Ly294002, by Merck Group (1 mentions)
Sb415286, by Merck Group (1 mentions)
Recombinant human pdgf bb, by Merck Group (1 mentions)
Fetal bovine serum fbs, by PromoCell (1 mentions)
Trypan blue reagent, by Merck Group (1 mentions)
α sma, by Merck Group (1 mentions)
Rosiglitazone, by Merck Group (1 mentions)
Polyvinylidene fluoride (pvdf), by Bio-Rad (1 mentions)
Ang 2, by Merck Group (1 mentions)
Fisetin, by Merck Group (1 mentions)
Jnk inhibitor sp600125, by Selleck Chemicals (1 mentions)
Mapk inhibitor pd98059, by Selleck Chemicals (1 mentions)
2 protocols using proliferating cell nuclear antigen antibody
1
Investigating PDGF-BB-Mediated Cell Proliferation
2
Molecular Mechanisms in Vascular Biology
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fisetin, AngII, rosiglitazone, and antibodies against ɑ smooth muscle actin (α-SMA) and osteopontin (OPN) were purchased from Sigma Co. (St. Louis, MO). Goat polyclonal antibody against PON2 was from Thermo Fisher Scientific (Waltham, MA). Proliferating cell nuclear antigen (PCNA) antibody was from Cell Signaling Technology (CST, USA). MAPK inhibitor PD98059, JNK inhibitor SP600125, and PPARγ inhibitor GW9662 were from Selleck Chemicals (Houston, Texas, USA). SDS-polyacrylamide gels were from Pierce (Rockford, IL). Polyvinylidene fluoride (PVDF) and Western blotting-related apparatus were from Bio-Rad (Hercules, CA). All serum, cell media, and antibiotics were purchased from Thermo Fisher Scientific (Waltham, MA). All organic solvents were from Solarbio Life Sciences (Shanghai, China).
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