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2 protocols using bax 5023

1

Apoptosis Signaling Pathway Analysis

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The following antibodies and reagents were used: Sirtuin Antibody Sampler Kit #9787T, NF‐κB p65 Antibody Sampler Kit #4767, Death Receptor Antibody Sampler Kit #8356, antibodies against cleaved PARP #5626, cleaved caspase‐3 #9664, cleaved caspase‐8 #8592, cleaved caspase‐9 #9509, Bak #6947, Bax #5023, Bcl‐2 #15071, and Bcl‐X #2764 (Cell Signaling Technology, Danvers, MA, USA); antibodies against HDAC3 #sc‐130319 and β‐actin #sc‐58673 (Santa Cruz Biotechnology, Dallas, TX, USA); nicotinamide (NAM); and ammonium pyrrolidine dithiocarbamate (PDTC) (Sigma‐Aldrich, St Louis, MO, USA).
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2

Molecular Mechanisms of Cell Apoptosis

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The hematoxylin and eosin (HE) staining kit (#C0105S), BCA kit (#P0010S), anti-rabbit Alexa Fluor488 immuno uorescence staining kit(#AF1873), anti-Bcl-2(#AF6285),anti-beta actin (#AF5003) antibody and propidium iodide were purchased from the Beyotime Institute of Biotechnology (Haimen, China). Anticaspase-3 (#14220), 9(#9502), anti-caspase-8 (#4709) and Bax (#5023) antibody were purchased from Cell Signaling Technology (Boston,USA). Anti-RhoA(#2117), anti-RhOC(#3430) and anti-ROCK1(#4035)antibodies were purchased from Proteintech (Chicago, IL, USA). The quantitative PCR (qPCR) kit was from Bio-Engineering Co., Ltd (Dalian, China). Trizol was purchased from Invitrogen (Carlsbad, CA, USA), and ω-3 PUFAs was from Huarui Pharmaceutical Co., Ltd (Wuxi, China). 5-FU was from JinYao Pharmaceutical Co., Ltd (Tianjin, China).
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