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Anti tnf α mab11

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Anti-TNF-α [MAb11] is a monoclonal antibody that binds to and neutralizes the pro-inflammatory cytokine Tumor Necrosis Factor-alpha (TNF-α). This product is intended for use in research applications.

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Lab products found in correlation

Sytox green, by Thermo Fisher Scientific (3 mentions) Anti cd3 sp34 2, by BD (2 mentions) Anti cd95 dx2, by BD (2 mentions) Anti ifn γ 4s b3, by BioLegend (2 mentions) Anti cd28 cd28.2, by Beckman Coulter (2 mentions) Anti cd8 2st8.5h7, by Bio-Rad (2 mentions) Anti il 2 mq1 17h12, by BioLegend (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Recombinant il 2, by Merck Group (1 mentions) Silica gel 60, by Merck Group (1 mentions) Anti ifnγ b27, by BD (1 mentions) Monensin, by BioLegend (1 mentions) Anti cd4 sk3, by BD (1 mentions) Anti cd8 sk1, by BD (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Brefeldin a, by Merck Group (1 mentions) Cytoflex, by Beckman Coulter (1 mentions) Cp 456773, by Merck Group (1 mentions) Uric acid, by Merck Group (1 mentions) Anti il 10 jes3 9d7, by BioLegend (1 mentions)

6 protocols using anti tnf α mab11

1

Multimodal Immune Modulation Assay

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The novel polyclonal Ig preparation, trimodulin (∼23% IgM, ∼21% IgA and
∼56% IgG), was kindly provided by Biotest AG (Dreieich, Germany).
Purified Escherichia coli LPS 0111:B4,
Staphylococcus aureus-derived lipoteichoic acid
(LTA), ConA and recombinant IL-2 were obtained from Sigma-Aldrich (St.
Louis, MO).
Fluorescently labelled Abs for flow cytometry were purchased from the
following manufacturers: anti-human leukocyte Ag–DR isotype (HLA-DR
G46-6-PE) and anti-TNF-α (mAB11) from BD Biosciences (San Jose, CA);
anti-TLR2 (CD282-PE) and anti-TLR4 (HTA125-PE) from eBioscience (San
Diego, CA); anti-cluster of differentiation 16 (CD16; 3G8-PE),
anti-CD64 (22-PC5), anti-CD11b (Bear1-PC5), anti-CD11c (BU15-PC5),
anti-CD3 (UCHT1-PC7), anti-CD4 (13B8.2-PC5) and anti-CD8
(SFCI21Thy2D3-ECD) from Beckman Coulter (Fullerton, CA); and anti-CD14
(My4; FITC, PC5 or PC7 conjugated) from Beckman Coulter (Indianapolis,
IN).
Duerr C., Bacher A., de Martin A., Sachet M., Sadeghi K., Baumann S., Heinz C, & Spittler A. (2019). The novel polyclonal Ab preparation trimodulin attenuates ex vivo endotoxin-induced immune reactions in early hyperinflammation. Innate Immunity, 25(6), 374-388.
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2

Mycobacterial Antigen Preparation and Immune Cell Assays

Cited 2 times
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Mammalian cell culture experiments were completed and Mycobacterium bovis BCG stocks prepared as reported previously (35 (link), 39 (link)). Whole-cell lysates of M. tuberculosis (MtbWL) (NR-14822) were obtained from BEI Resources. Soluble 4-hydroxy-but-2-enyl pyrophosphate (HMBPP; Echelon Bioscience, Salt Lake City, UT) was used for stimulation of γ9δ2 T cells as a control in inhibition assays. Interleukin 2 (IL-2; Hoffmann-LaRoche Inc., Basel, Switzerland) was used to expand γ9δ2 T cells. Anti-CD3 peridinin chlorophyll protein (PerCP; clone SK7), anti-αβ TCR fluorescein isothiocyanate (FITC; clone B3), anti-γδ TCR phycoerythrin (PE; clone 11F2), anti-CD4 PE-cy7 (clone RPA-T4), anti-CD8 V500 (clone RPA-T8), anti-Ki-67 (clone 35/Ki-67), anti-tumor necrosis factor alpha (anti-TNF-α; MAb11), anti-IFN-γ (B27), and anti-granzyme A (anti-GzmA; CB9) were all from BD Biosciences. Guava ViaCount Flex reagent (Millipore; 4700-0060) was used for accurate counting and discrimination of viable and nonviable cells from antigen-expanded cells. All chemicals for biochemical separations were obtained from Acros Organics (silica gel 60) and Sigma-Aldrich (chloroform and methanol). Alkaline phosphatase (AP) was obtained from Fisher Scientific (BP 80975).
Xia M., Hesser D.C., De P., Sakala I.G., Spencer C.T., Kirkwood J.S., Abate G., Chatterjee D., Dobos K.M, & Hoft D.F. (2016). A Subset of Protective γ9δ2 T Cells Is Activated by Novel Mycobacterial Glycolipid Components. Infection and Immunity, 84(9), 2449-2462.
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3

Multiparametric Flow Cytometry Immunophenotyping

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Surface stain monoclonal antibodies (mAbs) include: anti-CD4 [L200], anti-CD49d α4 integrin [9F10] and anti-CD95 [DX2] (BD Biosciences, San Jose, CA); anti-CD14 [TUK4], anti-CD20 [HI47] and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Grand Island, NY); anti-CD28 [CD28.2] (Beckman Coulter, Pasadena, CA); anti-CD8 [2ST8.5H7] (Custom, mAb from Serotec, conjugation kit from Invitrogen).Intracellular stain mAbs include: anti-CD3 [SP34-2] and anti-TNF-α [MAb11] (BD Biosciences); anti-IFN-γ [4S.B3] and anti-IL-2 [MQ1-17H12] (Biolegend, San Diego CA).
Kutzler M.A., Wise M.C., Hutnick N.A., Moldoveanu Z., Hunter M., Reuter M., Yuan S., Yan J., Ginsberg A., Sylvester A., Pahar B., Carnathan D., Kathuria N., Khan A.S., Montefiori D., Sardesai N.Y., Betts M.R., Mestecky J., Marx P, & Weiner D.B. (2015). Chemokine Adjuvanted Electroporated-DNA Vaccine Induces Substantial Protection from Simian Immunodeficiency Virus Vaginal Challenge. Mucosal immunology, 9(1), 13-23.
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4

Multiparametric Flow Cytometry Immunophenotyping

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Surface stain monoclonal antibodies (mAbs) include: anti-CD4 [L200], anti-CD49d α4 integrin [9F10] and anti-CD95 [DX2] (BD Biosciences, San Jose, CA); anti-CD14 [TUK4], anti-CD20 [HI47] and LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Invitrogen, Grand Island, NY); anti-CD28 [CD28.2] (Beckman Coulter, Pasadena, CA); anti-CD8 [2ST8.5H7] (Custom, mAb from Serotec, conjugation kit from Invitrogen).Intracellular stain mAbs include: anti-CD3 [SP34-2] and anti-TNF-α [MAb11] (BD Biosciences); anti-IFN-γ [4S.B3] and anti-IL-2 [MQ1-17H12] (Biolegend, San Diego CA).
Kutzler M.A., Wise M.C., Hutnick N.A., Moldoveanu Z., Hunter M., Reuter M., Yuan S., Yan J., Ginsberg A., Sylvester A., Pahar B., Carnathan D., Kathuria N., Khan A.S., Montefiori D., Sardesai N.Y., Betts M.R., Mestecky J., Marx P, & Weiner D.B. (2015). Chemokine Adjuvanted Electroporated-DNA Vaccine Induces Substantial Protection from Simian Immunodeficiency Virus Vaginal Challenge. Mucosal immunology, 9(1), 13-23.
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5

In Vitro SARS-CoV-2 T Cell Assay

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PBMCs were stimulated with peptide pools and expanded in vitro for 10 days as described before (23 (link)). Expanded T cell lines were stimulated for 5 hours at 37°C with or without SARS-CoV-2 peptide pools (2 μg/mL). After 1 hour, 10 μg/mL brefeldin A (MilliporeSigma) and 1× monensin (BioLegend) were added. Cells were stained with the yellow LIVE/DEAD fixable dead cell staining kit (Invitrogen, Thermo Fisher Scientific) and the surface markers anti-CD3 (SK7 or OKT3; BioLegend), anti-CD4 (SK3, BD Biosciences), and anti-CD8 (SK1, BD Biosciences). Cells were subsequently fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences) and stained with anti–IFN-γ (25723; R&D Systems) and anti–TNF-α (MAb11, BD Biosciences) antibodies and analyzed on a CytoFLEX (Beckman Coulter). Data were analyzed by FlowJo (BD Biosciences).
Samandari T., Ongalo J.B., McCarthy K.D., Biegon R.K., Madiega P.A., Mithika A., Orinda J., Mboya G.M., Mwaura P., Anzala O., Onyango C., Oluoch F.O., Osoro E., Dutertre C.A., Tan N., Hang S.K., Hariharaputran S., Lye D.C., Herman-Roloff A., Le Bert N, & Bertoletti A. (2023). Prevalence and functional profile of SARS-CoV-2 T cells in asymptomatic Kenyan adults. The Journal of Clinical Investigation, 133(13), e170011.
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6

Inflammasome Activation and Cytokine Profiling

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Uric acid, lipopolysaccharide (LPS) (O111:B4), and NLRP3 inflammasome inhibitor (CP-456773) were purchased from Sigma (St Louis, MO). Quant-iT PicoGreen dsDNA Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA). RPMI-1640, penicillin-streptomycin, and fetal bovine serum were obtained from WelGene (Daejeon, South Korea). For multi-color flow cytometry, the following antibodies were used: Fixable Viability Dye eFluor® 506, anti-CD3 (UCHT-1), anti-CD19 (HIB19), anti-CD56 (B159), and anti-IL-1RA (CRM17) (All from eBioscience, San Diego, CA, USA); anti-CD14 (MφP9), anti-IL-1β (AS10), and anti-TNF-α (Mab11) (All from BD Bioscience, San Jose, CA); anti-IL-10 (JES3-9D7) and anti-IL-8 (6217) (Both from Biolegend, San Diego, CA); anti-TGF–β1 (27232) (R&D Systems, Minneapolis, MN). For immunofluorescence, SYTOX Green and anti-MPO (4A4) were obtained from Thermo Fisher Scientific (Waltham, MA). Anti-NE (NP57) and anti-citrullinated histone H3 (rabbit polyclonal) were obtained from Santa Cruz Biotechnology Inc. (Dallas, Texas) and Abcam (Cambridge, MA), respectively.
Jeong J.H., Choi S.J., Ahn S.M., Oh J.S., Kim Y.G., Lee C.K., Yoo B, & Hong S. (2021). Neutrophil extracellular trap clearance by synovial macrophages in gout. Arthritis Research & Therapy, 23, 88.
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