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8454 uv vis spectrometer

Manufactured by Agilent Technologies
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The 8454 UV-vis spectrometer is a compact and versatile instrument designed for UV-visible spectroscopy analysis. It features a diode array detector and a wavelength range of 190 to 1100 nm, allowing for a wide range of applications. The 8454 provides accurate and reliable data collection for various sample types and analysis requirements.

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Lab products found in correlation

Av 400 spectrometer, by Bruker (1 mentions) Amazon sl ion trap mass spectrometer, by Bruker (1 mentions) F 4600 spectrophotometer, by Hitachi (1 mentions)

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UV-Vis spectrometer (27 citations) Spectrometers (10 citations) Cary 8454 UV-vis spectrometer (4 citations) Agilent 8454 UV-Vis spectrometer (2 citations)

4 protocols using 8454 uv vis spectrometer

1

Quantification of Peptide Thiols

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Samples for thiol quantification contains peptide and 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB, Acros Organics) in 100 mM TRIS pH 8.5. The samples are incubated for 15 min prior to quantification using ε412 nm = 14,150 M−1cm−1 on an Agilent 8454 UV-vis spectrometer.
Parambath S.M., Williams A.E., Hunt L.A., Selvan D., Hammer N.I, & Chakraborty S. (2021). A De Novo Designed Artificial Metallopeptide Hydrogenase: Insights into Photochemical Processes and the Role of Protonated Cys. ChemSusChem, 14(10), 2237-2246.
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2

Fluorescence-based DNAzyme Quantification

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Fluorescence measurements were conducted on a HORIBA fluorescence spectrophotometer (Fluro Max-P). In a typical experiment, 100 μL of DNAzyme and 100 μL of the extracted sample were sequentially added into a tube and measured immediately by a fluorescence spectrophotometer. Ultraviolet–visible (UV–vis) absorption spectra were obtained by taking sample solutions in a 1.0 cm quartz cuvette at 25 °C with total volumes kept at 100 μL and recorded on an Agilent Technologies, Cary 8454 UV–vis spectrometer. ICP–MS (NexION300Q, PE) was employed as a standard method to verify the quantities of zinc recovered from the standard metal solutions and the serum samples. Trace elements serum was used as a quality control material to monitor precision and trueness of laboratory measurement procedures. All samples were digested and dissolved with HNO3 before analysis and measured in triplicate.
Xing S., Lin Y., Cai L., Basa P.N., Shigemoto A.K., Zheng C., Zhang F., Burdette S.C, & Lu Y. (2021). Detection and Quantification of Tightly Bound Zn2+ in Blood Serum Using a Photocaged Chelator and a DNAzyme Fluorescent Sensor. Analytical chemistry, 93(14), 5856-5861.
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3

Characterization of Copper(II) Complexes

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Electronic absorbance spectra were collected at 298 K on an Agilent 8454 UV–vis spectrometer equipped with a diode-array detector. Sample solutions were prepared in deionized water and measured at pH 5.0 using concentrations of ~5 and ~0.5 mM for the copper(II) d–d and ligand π–π* transitions, respectively.
Vaughn B.A., Brown A.M., Ahn S.H., Robinson J.R, & Boros E. (2020). Is Less More? Influence of the Coordination Geometry of Copper(II) Picolinate Chelate Complexes on Metabolic Stability. Inorganic chemistry, 59(22), 16095-16108.
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4

Synthesis and Characterization of Fluorescent Molecular Probes

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Unless otherwise mentioned, all the reagents for the synthesis of the target compound were acquired from commercial suppliers and were used without further purification. All cations were added in the form of metal nitrates except for K+, Sn2+ and Hg2+ (all of their counter ions were chloride ions). Metal ions solutions (0.1 mol L−1) were prepared by dissolving their respective metal salts in deionized water. Necessary dilutions were made according to each experimental set up. NMR spectra were recorded on a Bruker AV400 spectrometer with deuterium generation of methanol (MeOD-d4) and dimethylsulfoxide (DMSO-d6) as solvents and tetramethylsilane (TMS) as an internal standard. Mass spectra were obtained using a Bruker Amazon SL ion trap mass spectrometer (ESI). The melting point was measured on a WRS-1B melting point apparatus. Absorption spectra were measured on an Agilent 8454 UV/vis spectrometer. Fluorescence spectra were recorded using a Hitachi F-4600 spectrophotometer. Photoirradiation was performed with an MUL-165 UV lamp and a MVL-210 visible lamp. Fluorescence quantum yield was measured with an Absolute PL Quantum Yield Spectrometer QYC11347-11.
Wang Z., Cui S., Qiu S, & Pu S. (2018). A highly selective fluorescence “turn-on” sensor for Ca2+ based on diarylethene with a triazozoyl hydrazine unit. RSC Advances, 8(51), 29295-29300.
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