Magna pure dna isolation system

Genomic DNA for genetic analysis was isolated from 400 μl EDTA blood with the MagNA Pure DNA-isolation system (Roche Diagnostics) according to the manufacturer’s instructions. Cytochrome (CYP) 2C9 genotyping was performed using the LightMix for the detection of human CYP2C9*2 and CYP2C9*3 variants (TIB MolBiol), LightCycler FastStart DNA Master Hybridisation Probes (Roche), and the LightCycler 2.0 capillary PCR machine, as previously described [12 (link)]. Allelic variants of CYP2C19 were determined with the Infiniti® CYP450 2C19+ assay (AutoGenomics, Carlsbad, CA, USA), as previously described [13 (link)]. Genotyping of CYP2C9 and CYP2C19 loss-of-function polymorphisms was available for 278 patients (92.4%) of group 1.

Peripheral blood from all cases and controls were used to isolate germline DNA from leukocytes. DNA extraction was performed automatically according to standard procedures using the Magnapure DNA isolation system (Roche Diagnostics, Mannheim, Germany).
For genome-wide genotyping we used the Afymetrix NspI chip as previously described [33] (link). For genotyping of selected SNPs in the NXC-VAL sample we employed custom Golden Gate protocols and Veracode genotyping assay (Illumina, San Diego, California USA) according to the manufacturer’s instructions.