Zona pellucida were removed from embryos with 3-4 min pronase treatment [0.5% w/v proteinase K (Sigma-Aldrich, P8811) in H-KSOM supplemented with 0.5% PVP-40 (Sigma-Aldrich, P0930)] at 37°C. Subsequently, embryos were incubated in serum containing anti-mouse antibody (Cedarlane, CL2301; lot no. 049M4847V) diluted 1:3 with KSOM for 30 min at 37°C. Following three brief washes in H-KSOM, embryos were incubated in guinea pig complement (Sigma-Aldrich, 1639; lot no. SLBX9353) diluted 1:3 with KSOM for 30 min at 37°C. Lysed outer cells were removed by mouth-pipetting with a narrow glass capillary to isolate the inner cells.
Cl2301
Manufactured by Cedarlane
The CL2301 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor capable of accommodating a variety of sample tubes. The centrifuge operates at a maximum speed of 6,000 rpm, enabling efficient separation of sample components.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cl2301
1
Zona Pellucida Removal and Embryo Isolation
2
Isolation of ICM from Embryos
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Zona pellucida were removed from embryos with 3-4 min pronase (0.5% w/v Proteinase K, Sigma P8811, in H-KSOM supplemented with 0.5% PVP-40) treatment at 37C Subsequently, embryos were incubated in serum containing anti-mouse antibody (Cedarlane, CL2301, Lot no. 049M4847V) diluted 1:3 with KSOM for 30 min at 37C. Following three brief washes in H-KSOM, embryos were incubated in guinea pig complement (Sigma, 1639, Lot no. SLBX9353) diluted 1:3 with KSOM for 30 min at 37C. Lysed outer cells were removed by mouth-pipetting with a narrow glass capillary to isolate the inner cells.
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