At the end of the plant growth promotion experiment, the plant height and the number of leaves were first recorded, and then the shoots and the roots from each pot were harvested separately. The root system was gently washed to remove the adherent sandy soil. The root samples were placed in clear plexiglass trays containing deionized water (approximately 1 cm depth) and scanned by the scanner (EPSON Perfection V800 Photo, Japan). WinRHIZO image analysis system (Regent Instruments, Quebec, QC, Canada) was used to assess the morphological traits, such as the root length, the root surface area, and the root volume. After having been scanned, the roots were collected, and a few root samples were used to analyze the DSE colonization rate (see section “DSE Colonization Analysis”). The fresh shoots and the remaining roots were dried at 70°C for at least 48 h to calculate the plant biomass.
Winrhizo image analysis system
Manufactured by Regent Instruments
Sourced in Canada
The WinRHIZO is an image analysis system designed to digitize, measure, and analyze root samples. It captures high-resolution images of root systems and provides precise measurements of various root parameters, such as length, surface area, and diameter distribution.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using winrhizo image analysis system
1
Root Morphology Analysis for Plant Growth
2
Root Morphology Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seedlings were harvested and root weight was recorded. Roots were scanned using an Epson Expression/STD 1600 scanner and personal computer with Intel Pentium III/500 CPU, 128 MB RAM, optimized for root analyses by Regent Instrument, Inc., and their length was analysed using the WinRHIZO image analysis system (Regent Instruments, Quebec, Canada). When scanning, each root sample was placed in a rectangular glass dish (300 × 200 mm) with ∼4–5 mm of water to untangle the roots and minimize root overlap. Three replicated roots were analysed for each treatment.
3
Comprehensive Plant Biomass Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Prior to harvest, the plant height and shoot branching in each replicate (three plants/pot) were recorded.
The roots and shoots in each replicate were subsequently harvested separately. The cleaned roots were scanned utilizing an Epson Perfection V800 Photo scanner. The root morphological parameters, including root length, root volume, root diameter and root surface area, were measured using the WinRHIZO image analysis system (Regent Instruments, Quebec, Canada). Fresh roots were subsequently collected and used to estimate the DSE colonization levels and indoleacetic acid (IAA) concentration. Similarly, fresh leaves were used to measure physiological parameters, such as superoxide dismutase (SOD) activity and glutathione (GSH) content. The remaining fresh shoots and roots were dried to a constant weight at 70 °C to con rm the plant biomass and the Na + and K + contents. Soil samples from each pot were sieved (2mm mesh size) and then split into two parts: one subsample was stored at -80 ℃ to analyze the community composition of soil microbes, and the other subsample was dried at room temperature to analyze soil physicochemical properties.
The roots and shoots in each replicate were subsequently harvested separately. The cleaned roots were scanned utilizing an Epson Perfection V800 Photo scanner. The root morphological parameters, including root length, root volume, root diameter and root surface area, were measured using the WinRHIZO image analysis system (Regent Instruments, Quebec, Canada). Fresh roots were subsequently collected and used to estimate the DSE colonization levels and indoleacetic acid (IAA) concentration. Similarly, fresh leaves were used to measure physiological parameters, such as superoxide dismutase (SOD) activity and glutathione (GSH) content. The remaining fresh shoots and roots were dried to a constant weight at 70 °C to con rm the plant biomass and the Na + and K + contents. Soil samples from each pot were sieved (2mm mesh size) and then split into two parts: one subsample was stored at -80 ℃ to analyze the community composition of soil microbes, and the other subsample was dried at room temperature to analyze soil physicochemical properties.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!