Glomax multi jr reader

For the East Asian cohort, 1883 study participants including 250 sporadic early-onset PD patients (onset age < 50 years), 602 sporadic late-onset PD patients (onset age R 50 years), 100 familial PD patients with positive family history (at least one other affected first-and/or second-degree relative with parkinsonism), and 931 age/gender/ethnicity-matched controls, were recruited from the intensity of an individual mitochondrion was normalized to that of the adjacent cytoplasmic region. Six larvae were used for each genotype.
Detection of ATP Level ATP level was measured using a luciferase-based bioluminescence assay (ATP Determination Kit, Life Technologies) as previously described (Tsai et al., 2014) . For each experiment, 5-day-old adult flies were homogenized in 100 mL lysis buffer (6 M guanidine-HCl, 100 M Tris pH 8.0, and 4 mM EDTA). The extracts were boiled for 5 min, placed on ice for 5 min, and centrifuged at 20,000 g for 15 min. The supernatant was then diluted to 1:500 in reaction buffer (provided by the kit) and luciferase was added for 1 min. Luminescence was immediately measured using a Glomax Multi Jr. Reader (Promega). Each reading was normalized to protein concentration measured by bicinchoninic acid (BCA) assay (Thermo Scientific). Five adults were used for each experiment and total there were 6 independent experiments.