Anti acetylated lysine 9441

Anti-HSF1 (51034-1-AP), anti-HSP90 (13171-1-AP), anti-HSP70 (10995-1-AP), anti-HSP40 (13174-1-AP), and anti-GAPDH (10494-1-AP) were all purchased from ProteinTech Group while anti-HSF2 (ab126252), anti-HSF1 ser307 (ab75905), and anti-HSF1 ser326 (ab115702) were purchased from Abcam Inc. Anti-SIRT1 was purchased from Cell signaling technology while anti-HSBP1 was purchased from MyBiosource. The horseradish peroxidase–conjugated secondary antibodies were purchased from BioRad Laboratories Inc. Real-time PCR primers for HSF1 (F-ACAGTGTCACCCGGCTGTTG and R-GACTGCACCAGTGAGATGAGGAA), HSF2 (F-GCAGTGTTGTTCAACATGTGTCAG and R-AGTTCCCATCCAGGAATGCAAG), and endogenous control B2M (F-ATGGGAAGCCGAAC-ATACTG and R-CAGTCTCAGTGGGGGTGAAT) were purchased from Eurofins MWG Genomics LLC and HSE probe for electrophoretic mobility shift assay (EMSA) was purchased from Integrated DNA Technologies. Anti-acetylated lysine (9441) was purchased from Cell Signaling Technology while anti-rabbit IgG HRP (5220-0337) was purchased from Sera Care.

Anti-HA (3724), anti-GSDMD (39754) and anti-Acetylated Lysine (9441) antibodies were purchased from Cell Signaling Technology. Anti-GAPDH antibody, HRP-labeled goat anti-mouse IgG and goat anti-rabbit IgG were from Hangzhou Fudebio. Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads (10004D), anti-Myc (C3956) antibodies and LPS (O111:B4, L2630) were purchased from Sigma-Aldrich. Anti-FLAG antibody (rabbit source), Goat pAb to MS IgG (Chromeo 555, ab60316), Dnk pAb to Rb IgG (Alexa Fluor 647, ab150075) fluorescent secondary antibodies were obtained from Abcam. Nigericin, Pam3CSK4 (TLRL-PMS), poly(dA:dT) (TLRL-PATN) and flagellin (TLRL-EPSTFLA) were purchased from InvivoGen. ELISA kits for analysis of human IL-1β, mouse IL-1β, mouse IL-6 and mouse TNF-α were purchased from MultiSciences. CytoTox 96 LDH-release assay kit (G1780) was from Promega. Efficient eukaryotic transfection reagent VigoFect was purchased from Vigorous Biotechnology (Beijing), Lipofectamine 2000 transfection reagent was obtained from Invitrogen while Lipo8000 transfection reagent was obtained from Beyotime Biotechnology. Trichostain A (HY-15144), Nicotinamide (HY-B0150), LMK-235(HY-18998) and okadaic acid (HY-N6785) were purchased from MCE.