Camptothecin cpt

The ATRi VE822 and the EZH2 inhibitor GSK-126 were purchased from Selleck, and the ATM inhibitor KU55933 and the DNA-PK inhibitor NU7441 from Merck Millipore. The Mre11 inhibitor Mirin, 5-bromo-2′-deoxyuridine (BrdU), 5-chloro-2′-deoxyuridine (CldU), 5-iodo-2′-deoxyuridine (IdU) and chloroquine (CQ) were purchased from Sigma-Aldrich. Cisplatin was purchased from Wako, and camptothecin (CPT) was purchased from Abcam.

CuO NPs were obtained from Intrinsiq Materials Ltd (Farnborough, UK) and were supplied by the Nanovalid consortium (http://www.nanovalid.eu/). Acetonitrile (ACN) for LC-MS was purchased from VWR (Radnor, PA, USA). High-purity water (H₂O) was produced using a Milli-Q Integral three purification system from Merck Millipore (Darmstadt, Germany). Standard substances used for identification were obtained from Merck (amino acids). Staurosporine (STS) was purchased from Proteinkinase.de (Kassel, Germany), camptothecin (CPT) from Abcam (Cambridge, UK), and rhTNF-α from Immunotools (Friesoythe, Germany). SYBR Green Supermix was purchased from Bio-Rad (Munich, Germany), RevertAid HMinus M-MulV reverse transcriptase from Fermentas (St. Leon-Roth, Germany), TRIzol reagent from Invitrogen, IL-8 ELISA kits from PeproTech, while Celltiter-Blue® (CTB) Cell Viability Assay was purchased from Promega (Madison, WI, USA), foetal calf serum (FCS) from PAA (Pasching, Austria). All other substances used were obtained from Sigma-Aldrich (St. Louis, MO, USA).