All the chemicals were procured from Sigma Aldrich with product numbers provided below. Gluteraldehyde (2.5%, Sigma Aldrich, St. Louis, MO, USA, #G5882), osmium tetroxide (1%, Sigma Aldrich, #75633), 0.1 M phosphate buffer solution (Sigma Aldrich, #P5244), ethanol (Sigma Aldrich, #E7023), propylene oxide (Sigma Aldrich, #82320), epoxy embedding medium 812 substitute (Sigma Aldrich, #45345), epoxy embedding medium hardener DDSA (Sigma Aldrich, #45346), epoxy embedding medium hardener MNA (Sigma Aldrich, #45347), epoxy embedding medium accelerator DMP 30 (Sigma Aldrich, #45348), oil, polycaprolactone (Sigma-Aldrich, #440744), dichloromethane (Sigma Aldrich, #270997), N-N-Dimethylformamide (Sigma Aldrich #319937).
Dmp 30
Manufactured by Merck Group
Sourced in United States
The DMP 30 is a laboratory equipment product from Merck Group. It is designed to perform differential pressure measurements, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Propylene oxide, by Merck Group (2 mentions)
Osmium tetroxide, by Merck Group (2 mentions)
Polycaprolactone, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Dichloromethane, by Merck Group (2 mentions)
N n dimethylformamide (dmf), by Merck Group (2 mentions)
Pyridine, by Merck Group (2 mentions)
Gluteraldehyde, by Merck Group (1 mentions)
0.1 m phosphate buffer solution, by Merck Group (1 mentions)
Phosphate buffer solution (pbs), by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Orius sc1000 ccd camera, by Ametek (1 mentions)
Uc7 ultramicrotome, by Leica camera (1 mentions)
Jem 1400 electron microscope, by JEOL (1 mentions)
4 protocols using dmp 30
1
Electron Microscopy Sample Preparation
2
Electron Microscopy Sample Preparation
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The following materials were used in this study and were procured from Sigma-Aldrich: pyridine (#270970), 2.5% of glutaraldehyde solution (#G5882), phosphate buffer solution (#P5244), 1% osmium tetroxide (#75633), ethanol (#E7023), propylene oxide (#82320), epoxy embedding medium 812 substitute (#45345), epoxy embedding hardener DDSA (#45346), epoxy medium accelerator DMP 30 (#45348), epoxy embedding hardener MNA (#45347), acetic acid (#695092), polycaprolactone MW = 80000 g/mol (#440744), and formic acid (#1.10854).
3
Transmission Electron Microscopy Sample Preparation
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All chemicals were acquired from Sigma Aldrich, and their product numbers are listed below. Glutaraldehyde (Sigma Aldrich, #G5882), osmium tetroxide (1%, Sigma Aldrich, #75633), Phosphate buffer solution (Sigma Aldrich, #P5244), ethanol (Sigma Aldrich, #E7023), propylene oxide (Sigma Aldrich, #82320), epoxy embedding medium 812 substitute (Sigma Aldrich, #45345), polycaprolactone (Sigma-Aldrich, #440744), dichloromethane (Sigma Aldrich, #270997), N-N-Dimethylformamide (Sigma Aldrich, #319937), epoxy embedding medium hardener DDSA (Sigma Aldrich, #45346), epoxy embedding medium hardener MNA (Sigma Aldrich, #45347), epoxy embedding medium accelerator DMP 30 (Sigma Aldrich, #45348), polycaprolactone (Sigma-Aldrich, ##440744), acetic acid (#270725), formic acid (90%, #110854), pyridine (Sigma-Aldrich #270970).
4
Transmission Electron Microscopy Preparation
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Cells were rinsed with 0.15 M sodium cacodylate and with 2.5% glutaraldehyde for 1 h. After fixation, the cells were rinsed with 0.15 M sodium cacodylate and post-fixed with 1.5% osmium tetroxide for 1 h. After further rinsing, the samples were dehydrated in increasing concentrations of ethanol (50%, 70%, 90% and 100% vol/vol). The cells were gradually infiltrated with increasing concentrations of epoxy resin (30%, 50%, 70% vol/vol in ethanol) for a minimum of 3 h per concentration. The samples were then incubated overnight in pure epoxy resin before continuing with a two-step incubation in 2,4,6-Tris(dimethylamino-methyl)phenol (DMP30; Sigma-Aldrich)-epoxy resin, first for 3 h and then for 24 h at 60°C to polymerise the samples en bloc. Ultra-thin sections of 80 nm were cut from the blocks using a UC7 ultramicrotome (Leica), placed on grids, post-stained with uranyl acetate for 30 min and with lead citrate for 20 min. Sections were examined using a JEM-1400 Electron Microscope (JEOL Ltd.) operating at 120 kV accelerating voltage. Digital images were acquired using a Gatan SC1000 Orius CCD camera with a dedicated imaging software (GataDigitalMicrographTM).
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