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Pparα sc 9000

Manufactured by Santa Cruz Biotechnology
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PPARα (sc-9000) is a lab equipment product offered by Santa Cruz Biotechnology. It is a nuclear receptor that functions as a transcription factor. The core function of PPARα is to regulate the expression of genes involved in lipid and carbohydrate metabolism.

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Lab products found in correlation

Trypsin edta, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin mixed antibiotics, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions) P hsl s563, by Cell Signaling Technology (1 mentions) P ampkαt172, by Cell Signaling Technology (1 mentions) Adiponectin, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Anti mouse igg nxa931, by Cytiva (1 mentions) Perilipin 1, by Cell Signaling Technology (1 mentions) Pcreb s133, by Cell Signaling Technology (1 mentions) Ha tag, by Cell Signaling Technology (1 mentions) P ampkα thr172, by Cell Signaling Technology (1 mentions) P38 mapk, by Cell Signaling Technology (1 mentions) Probucol, by Merck Group (1 mentions) P foxo1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

PPARα (67 citations) Sc-9000 (10 citations)

4 protocols using pparα sc 9000

1

Lipid Metabolism Pathway Regulation

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Dulbecco’s modified Eagle’s medium (DMEM), L-glutamine, penicillin -streptomycin mixed antibiotics, fetal bovine serum (FBS), trypsin-EDTA, and phosphate-buffered saline (PBS) were purchased from Gibco/BRL (Gaithersburg, MD, USA). Primary antibodies against SREBP-1, SREBP-2, HMG-CoAR (HMGCR; sc-271595), AMPK (sc-74461), CPTI (sc-20669), and PPARα (sc-9000) were obtained from Santa Cruz (Santa Cruz, CA, USA). Monoclonal antibodies against phosphor-AMPK (#2535), FASN (#3180) and α-tubulin were obtained from Cell Signaling Technology Inc (Beverly, MA, USA).
Yang T.Y., Yu M.H., Wu Y.L., Hong C.C., Chen C.S., Chan K.C, & Wang C.J. (2022). Mulberry Leaf (Morus alba L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation. Nutrients, 14(22), 4808.
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2

AMPK Pathway Regulation in Lipid Metabolism

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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin mixed antibiotics, L-glutamine, Dulbecco’s phosphate-buffered saline (PBS), and Trypsin–EDTA were provided by Gibco/BRL (Gaithersburg, MD, USA). Monoclonal antibodies against AMPK (sc-74461), CPTI (sc-20669), SREBP1, SREBP2, HMG-CoA reductase (HMGCR; sc-271595), and PPARα (sc-9000) were obtained from Santa Cruz (Santa Cruz, CA, USA). Cell Signaling Technology Inc (Beverly, MA, USA) provided antibodies against phosphor-AMPK (#2535) and FASN (#3180). The enhanced chemiluminescence kit was purchased from Amersham Life Sciences (Amersham, UK). Polyclonal antibodies against actin, OA, and 5-CQA and other chemical reagents were procured from Sigma Chemical (St. Louis, MO, USA).
Yu M.H., Hung T.W., Wang C.C., Wu S.W., Yang T.W., Yang C.Y., Tseng T.H, & Wang C.J. (2021). Neochlorogenic Acid Attenuates Hepatic Lipid Accumulation and Inflammation via Regulating miR-34a In Vitro. International Journal of Molecular Sciences, 22(23), 13163.
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3

Adiponectin Regulation of Lipolysis

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Immunoblots were conducted on 3 to 7 samples randomly chosen within each experimental cohort with antibodies against HSL (#4107 or #18381), p-HSL (S563) (#4139), p-HSL (S565) (#4137), p-HSL (S660) (#4126), Perilipin-1 (#9349), p-AMPKα (T172) (#2535), AMPKα (#2532), p-CREB (S133) (#9198), CREB (#9197), p-(Ser/Thr) PKA substrate (#9621), HA-tag (#3724), Adiponectin (#2789) and β-actin (#4970) from Cell Signaling (Denvers, MA); PHLPP1, (A300-660A), PHLPP2 (A300-661A) from Bethyl Laboratories, Inc (Montgomery, TX); PPARα (SC-9000) from Santa Cruz Biotechnology (Dallas, TX). Mouse serum was neutralized by an Adiponectin antibody (ab3455) from Abcam46 (link). All primary antibodies were used at a dilution of 1:1000. HRP-linked anti-rabbit IgG (NA934) and anti-mouse IgG (NXA931) were purchased from Amersham and used at a dilution of 1:5000. Alexa Fluor 594 anti-Rabbit IgG (A32754) was purchased from ThermoFisher and used at a dilution of 1:1000.
Kim K., Kang J.K., Jung Y.H., Lee S.B., Rametta R., Dongiovanni P., Valenti L, & Pajvani U.B. (2021). Adipocyte PHLPP2 inhibition prevents obesity-induced fatty liver. Nature Communications, 12, 1822.
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4

Immunoblotting Protocol for Cellular Signaling

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The primary antibodies FGF-2 (sc-79), IL-6 (sc-7920), MMP-9 (sc-6841), MMP-2 (sc-13595), PPAR-α (sc-9000), α-tubulin (sc-5286), β-actin (sc-47778), TNF-α (sc-1350) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); AMPKα (#2532), p-AMPKα (Thr172) (#2535), p-FoxO1 (#9464), p38 MAPK (#9212) and p-p38 MAPK (Thr180/Tyr182) (#9211) from Cell Signaling Technology (Danvers, MA, USA). All other reagents and chemicals, including Probucol, were from Sigma Aldrich (St. Louis, MO, USA) unless otherwise mentioned.
Dumeus S., Shibu M.A., Lin W.T., Wang M.F., Lai C.H., Shen C.Y., Lin Y.M., Viswanadha V.P., Kuo W.W, & Huang C.Y. (2018). Bioactive Peptide Improves Diet-Induced Hepatic Fat Deposition and Hepatocyte Proinflammatory Response in SAMP8 Ageing Mice. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(5).
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