Protein samples were run on SDS-PAGE gels and transferred to nitrocellulose membranes. The membranes were blocked with 5% non-fat milk, rinsed with TBST, and then incubated with primary and/or secondary antibodies. The primary antibodies used were anti-FLAG-HRP (Sigma, 1:5000), anti-HA-HRP (Roche, 1:5000), anti-GFP (Roche, 1:1000), anti-actin (Proteintech, 1:5000), anti-tubulin (Servicebio, 1:5000), and anti-H3 (Abcam, 1:1000). All antibodies were in 3% BSA in 1x TBST buffer. Chemiluminescence images were taken after adding ECL substrate with ImageQuant LAS4000 (GE).
Anti tubulin
Manufactured by Wuhan Servicebio Technology
Sourced in China
Anti-tubulin is a laboratory product used to detect and quantify tubulin, a key structural component of the cytoskeleton in eukaryotic cells. It is a reagent commonly used in cell biology and biochemistry research.
Automatically generated - may contain errors
Lab products found in correlation
Anti actin, by Proteintech (2 mentions)
Anti ha hrp, by Roche (2 mentions)
Anti h3, by Abcam (2 mentions)
Anti flag hrp, by Merck Group (2 mentions)
Imagequant las 4000, by GE Healthcare (2 mentions)
Anti gfp, by Roche (2 mentions)
Hrp labeled goat anti rabbit igg h l, by Wuhan Servicebio Technology (1 mentions)
Anti gapdh, by Wuhan Servicebio Technology (1 mentions)
Anti cd206, by Abcam (1 mentions)
Prism 5, by GraphPad (1 mentions)
4 protocols using anti tubulin
1
Protein Detection via Western Blotting
2
Western Blotting of Proteasomal and Signaling Proteins
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Western blotting was performed as reported previously [51 (link)]. The following antibodies were used: anti-PSMD1 (#A16420; rabbit polyclonal antibody; ABclonal; 1:1000 dilution), anti-PSMD2 (#14748-1-AP; rabbit polyclonal antibody; Proteintech; 1:1000 dilution), anti-ASK1 (#A6274; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti- p-ASK1 (#AP0394; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-AKT (#A11027; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p-AKT (#AP0140; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p38 (#A10832; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p-p38 (#AP0297; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-JNK1/2 (#A11119; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-p-JNK1/2 (#AP0631; rabbit polyclonal antibody; ABclonal; 1:2000 dilution), anti-GAPDH (Servicebio, Wuhan, China; 1:2000 dilution), anti-Tubulin (Servicebio, Wuhan, China; 1:2000 dilution), HRP-labeled Goat Anti-Rabbit IgG (H + L) (#GB23303-1, Servicebio, Wuhan, China), and HRP-labeled Goat Anti-Mouse IgG (H + L) (#GB23301, Servicebio, Wuhan, China).
3
Protein Detection via Western Blotting
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Protein samples were run on SDS-PAGE gels and transferred to nitrocellulose membranes. The membranes were blocked with 5% non-fat milk, rinsed with TBST, and then incubated with primary and/or secondary antibodies. The primary antibodies used were anti-FLAG-HRP (Sigma, 1:5000), anti-HA-HRP (Roche, 1:5000), anti-GFP (Roche, 1:1000), anti-actin (Proteintech, 1:5000), anti-tubulin (Servicebio, 1:5000), and anti-H3 (Abcam, 1:1000). All antibodies were in 3% BSA in 1x TBST buffer. Chemiluminescence images were taken after adding ECL substrate with ImageQuant LAS4000 (GE).
4
Immunohistochemical Analysis of Tumor Tissue
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The tumor tissue samples were fixed with 4% paraformaldehyde for 48 h. The paraffinembedded sections (5-µm thick) were prepared and dehydrated in a graded series of alcohol. The sections were stained with hematoxylin-eosin (H&E) and mounted with neutral gum. To detect tubulin and CD206 expression in tumor tissue samples, the paraffin-embedded sections were dewaxed in xylene and dehydrated in ethanol, followed by antigen retrieval with ethylenediaminetetraacetic acid (EDTA). The sections were blocked with 5% bovine serum albumin. The protein expression of tubulin and CD206 were detected using anti-tubulin (Servicebio) and anti-CD206 (Abcam), respectively. The results were examined under Pannoramic scan at a magnification of 20×. Images were acquired using a CaseViewer system.
Statistical Analysis Data were presented as the means ± stan- dard deviation. Statistical analysis was performed using the Prism 5 software (GraphPad, San Diego, CA, U.S.A.). The two groups were compared using the two-tailed unpaired Student t-test. A p value <0.05 was considered statistically significant.
Statistical Analysis Data were presented as the means ± stan- dard deviation. Statistical analysis was performed using the Prism 5 software (GraphPad, San Diego, CA, U.S.A.). The two groups were compared using the two-tailed unpaired Student t-test. A p value <0.05 was considered statistically significant.
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