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4 protocols using polypyrrole ppy

1

Collagen-Quercetin-DMSO Biocatalytic Hydrogel

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Collagen (Col) (Marine fish extract), Quercetin (Qur), Dimethyl sulfoxide (DMSO)were obtained from Sigma Aldrich, China. Polypyrrole (PPy) was obtained from Sigma Aldrich, China. All chemicals were obtained from analytical grade and were used as purchased without further purification. Double-distilled (DD) water was used throughout the experiments.
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2

Graphene Oxide-based Catalytic Materials

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Graphene oxide (GO, made from graphite flake, GO’, small flakes and dry platelets) was obtained from XF NANO, INC and graphene supermarket, respectively. Hydrazine monohydrate (N2H4·H2O, 80%) was purchased from Guangdong Guanghua Sci-Tech Co., Ltd (JHD). (NH4)2MoS4, Polypyrrole (PPy,) and Pt/C (10% Pt) were obtained from Sigma-Aldrich. N, N-dimethylformamide (DMF, ≥99.5%) and KOH were purchased from Sinopharm Chemical Reagent Co., Ltd. Sulfuric acid (H2SO4, 95–98%) and ethanol (99.9%) was acquired from Beijing Chemical Works. All aqueous solutions were prepared with doubly distilled water.
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3

Polymer Composites Synthesis and Characterization

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Polyvinyl acetate (PVAc) and polypyrrole (PPy) were provided by Sigma–Aldrich (Madrid, Spain). Gallic acid (C6H2(OH)3CO2H) (GA) was purchased from Sigma–Aldrich (Madrid, Spain). Potassium chloride (KCl), disodium-hydrogen phosphate (Na2HPO4), potassium dihydrogen phosphate (KH2PO4), and sodium chloride (NaCl) were purchased from Panreac Applychem (Barcelona, Spain). CO2 with a minimum purity of 99.8% was supplied by Linde (Barcelona, Spain).
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4

Enzymatic Assay for Xanthine Oxidase

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Xanthine oxidase, hypoxanthine, and polypyrrole (PPy) were obtained from Sigma-Aldrich (St. Louis, MO). Blocker Casein in phosphate buffered saline (PBS) buffer and Blocker BSA (bovine serum albumin; 10%) in PBS and Dulbecco's PBS (DPBS) buffer were purchased from Thermo Scientific (Tustin, CA). Deionized (DI) water was generated using a Barnstead Smart2Pure water purification system (Barnstead, Van Nuys, CA). Xanthine oxidase was resolved using 10% BSA in PBS and serially diluted in PBS buffer. All remaining biochemicals were used without further purification.
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