T butyl methyl ether

Manufactured by Merck Group

Sourced in United States

T-butyl methyl ether is a colorless, volatile, and flammable organic compound. It is a common solvent used in various laboratory applications due to its chemical properties.

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Tert-butyl methyl ether (t-BME) (7 citations)

7 protocols using t butyl methyl ether

Armodafinil Pharmacokinetic Analysis

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Armodafinil, deuterium water (D₂O, 99.9% purity), methanol—d₁ (MeOD, 99.9% purity), t-butylmethyl ether (TBME), N,N,N-triethylamine (TEA), acetonitrile (MeCN), formic acid (HCOOH) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). The used solvents were all of LC-MS grade.

Grocholska P., Wieczorek R, & Bąchor R. (2022). Preparation of Deuterium-Labeled Armodafinil by Hydrogen–Deuterium Exchange and Its Application in Quantitative Analysis by LC-MS. Metabolites, 12(7), 578.

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Copolymer Synthesis and Paclitaxel Encapsulation

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2-Methylcyclohexanon (99.0%), potassium peroxymonosulfate
(Oxone), sodium bicarbonate (99.0%), calcium hydride (95%), stannous
octonate (95%), PEG m ethyl ether (MePEG-OH, MW ∼5000), and
ε-caprolactone (97%) were all purchased from Sigma-Aldrich and
used for the synthesis of copolymers. NaCl (Bio Basic Canada, 99.9%),
KCl (Caledon, 99.0%), Na₂HPO₄ (Bio Basic Canada,
98.0%), and KH₂PO₄ (Caledon, 99.0%) were used
to prepare phosphate buffered saline (PBS, pH = 7.4). Methanol (Fisher
Chemical, 99.9%), methylene chloride (Fisher Chemical, 99.9%), toluene
(Caledon, 99.5%), ethyl ether (VWR, 99%), dimethylformamide (DMF,
Caledon, 99.8%), acetonitrile (Caledon, HPLC grade), t-butyl methyl ether (Sigma-Aldrich, HPLC grade), and dichloromethane
(DCM, EMD, HPLC grade) were used as received without further purification.
Paclitaxel (99.0%) was purchased from Polymed Inc.

Xu Z., Lu C., Lindenberger C., Cao Y., Wulff J.E, & Moffitt M.G. (2017). Synthesis, Self-Assembly, and Drug Delivery Characteristics of Poly(methyl caprolactone-co-caprolactone)-b-poly(ethylene oxide) Copolymers with Variable Compositions of Hydrophobic Blocks: Combining Chemistry and Microfluidic Processing for Polymeric Nanomedicines. ACS Omega, 2(8), 5289-5303.

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Coriander Seed Oil Extraction

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The vegetable oil of C. sativum fruits was isolated via twin-screw extrusion at LCA via a previously reported procedure (Uitterhaegen et al. 2015 (link)). Trimethylsulfonium hydroxide (TMSH), t-butyl methyl ether (TBME), hydrochloric acid (HCl), hexane, sodium citrate tribasic dihydrate, ammonium chloride (NH₄Cl), potassium phosphate monobasic (KH₂PO₄), potassium phosphate dibasic (K₂HPO₄), magnesium sulfate heptahydrate (MgSO₄∙7H₂O), calcium chloride dihydrate (CaCl₂∙2H₂O), sodium, 4-dimethylaminopyridine (DMAP), Sudan III and diethyl ether were purchased from Sigma-Aldrich (Belgium). Yeast extract was purchased from Difco (Belgium). Sodium hydroxide (NaOH), dry methanol, acetic anhydride and sodium triacetoxyborohydride [NaBH(OAc)₃] were purchased from Acros Organics (Belgium). Magnesium sulfate (MgSO₄) and sodium bicarbonate (NaHCO₃) were purchased from Fisher Scientific (Belgium). Ethyl acetate and tetrahydrofuran (THF) were purchased from Chem-Lab (Belgium). Glucose monohydrate was purchased from Cargill (Belgium). Sodium chloride (NaCl) was purchased from Colruyt (Belgium).

Delbeke E.I., Everaert J., Uitterhaegen E., Verweire S., Verlee A., Talou T., Soetaert W., Van Bogaert I.N, & Stevens C.V. (2016). Petroselinic acid purification and its use for the fermentation of new sophorolipids. AMB Express, 6, 28.

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Oxidation of 5-Methoxymethylfurfural

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P. pastoris MOX (EC 1.1.3.13), t-butyl-methyl-ether, MMFA, FDCA, N,O-bis(trimethylsilyl)-trifluoroacetamide (BSTFA) and ²H₂O were purchased from Sigma-Aldrich (Saint Louis, MO, USA). MMF (= 5-[methoxymethyl]-2-furancarboxaldehyde) was bought from AK Scientific, Inc (Union City, CA, USA). FFCA was purchased from TCI America (Portland, OR, USA). AmplexRed^® and HRP were obtained from Invitrogen (Walthem, MA, USA). H₂O₂ and DMSO-d6 were from Merck (Darmstadt, Hessen, Germany).

Carro J., Fernández-Fueyo E., Fernández-Alonso C., Cañada J., Ullrich R., Hofrichter M., Alcalde M., Ferreira P, & Martínez A.T. (2018). Self-sustained enzymatic cascade for the production of 2,5-furandicarboxylic acid from 5-methoxymethylfurfural. Biotechnology for Biofuels, 11, 86.

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Peptide Synthesis Reagents and Assays

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Glu- and Lys-plasminogen, plasmin, low molecular weight urokinase and single or double chain t-PA were from Enzyme Research Laboratories (South Bend, IN) or Innovative Research (Novi, MI). Activated partial thromboplastin time (aPTT; STA-PTT Automate) and prothrombin time (PT; Neoplastine CI Plus) reagents were from Diagnostica Stago (Asnières, France). S-2251 (H-D-Valyl-L-leucyl-L-lysinep-Nitroaniline dihydrochloride) was obtained from Diapharma (West Chester, OH). ε-Aminocaproic acid (ε-ACA), 4-mercaptophenylacetic acid, trifluoroacetic acid (TFA), triisopropylsilane (TIS), diisopropylcarbodiimide (DIC), 3,6-Dioxa-1,8-octane-dithiol (DODT), 4-methylpiperidine, methyl t-butyl ether (MTBE) and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were from Sigma (Saint Louis, MO). HBTU, N,N-diisopropylethylamine (DIPEA) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) were from Chem-Impex International (Wood Dale, IL). Fmoc-amino acid for peptide synthesis were from Midwest Biotech Inc (Fishers, IN). Dimethylformamide (DMF) and N-methylpyrrolidone (NMP) were from AGTC Bioproducts (Framingham, MA).

Assumpção T.C., Mizurini D.M., Ma D., Monteiro R.Q., Ahlstedt S., Reyes M., Kotsyfakis M., Mather T.N., Andersen J.F., Lukszo J., Ribeiro J.M, & Francischetti I.M. (2018). Ixonnexin from Tick Saliva Promotes Fibrinolysis by Interacting with Plasminogen and Tissue-Type Plasminogen Activator, and Prevents Arterial Thrombosis. Scientific Reports, 8, 4806.

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Lipidomic Analysis by LC-MS

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LC/MS (Merck KGaA, Darmstadt, Germany) grade Water (LiChrosolv), Acetonitrile (ACN), Methanol (MeOH), and Isopropanol (IPA) all (Carlo Erba, Milan, Italy), ammonium fluoride, ammonium acetate, methyl-t-butyl ether (MTBE), and chloroform (Chlo) were purchased from Sigma-Aldrich (Sigma-Aldrich GmbH, Hamburg, Germany). Internal standard (IS) was Splash Lipidomix (Avanti Polar, Alabaster, AL, USA): mixture with known concentration of the following lipids [nmol/mL]: PC 15:0_18:1(d7) [212.6]; PE 15:0_18:1(d7) [7.0]; PS 15:0_18:1(d7) [6.6]; PG 15:0_18:1(d7) [40.5]; PI 15:0_18:1(d7) [12.1]; PA 15:0_18:1(d7) [10.5]; LPC 18:1(d7) [47.3]; LPE 18:1(d7) [10.3]; CE 18:1(d7) [532.2]; MG 18:1(d7) [5.5]; DG 15:0_18:1(d7) [17.0]; TG 15:0_18:1(d7)_15:0 [67.8]; SM 18:1;2O/18:1(d9) [40.7]; Cholesterol(d7)[254.2].

Alabed H.B., Gorello P., Pellegrino R.M., Lancioni H., La Starza R., Taddei A.A., Urbanelli L., Buratta S., Fernandez A.G., Matteucci C., Caniglia M., Arcioni F., Mecucci C, & Emiliani C. (2023). Comparison between Sickle Cell Disease Patients and Healthy Donors: Untargeted Lipidomic Study of Erythrocytes. International Journal of Molecular Sciences, 24(3), 2529.

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Solvent Preparation for LC-MS Analysis

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Acetonitrile (LC/MS grade) and propan-2-ol (LC/MS grade) were purchased from Biosolve BV (Valkenswaard, The Netherlands). Methyl t-butyl ether (>99.8%), methanol (99.9%), ammonium formate (>99.0%), lithium formate monohydrate (98%), and sodium formate (>99.0%,) were obtained from Sigma-Aldrich (St. Luis, MO, USA). Chloroform (99.8%) stabilized by ~1% ethanol from Penta (Chrudim, Czech Republic) was purified by distillation.

Cudlman L., Machara A., Vrkoslav V., Polášek M., Bosáková Z., Blanksby S.J, & Cvačka J. (2023). Characterization of Triacylglycerol Estolide Isomers Using High-Resolution Tandem Mass Spectrometry with Nanoelectrospray Ionization. Biomolecules, 13(3), 475.

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