Bacillus subtilis CICC10012 (B. subtilis), Salmonella enterica WX29 (S. enterica), Staphyloccocus aureus CICC10201 (S. aureus), Bacillus cereus ATCC11778 (B. cereus), Escherichia coli CICC10899 (E. coli), Pseudomonas brenneri CICC10271 (P. brenneri) were purchased from the China Center of Industrial Culture Collection (Beijing, China) and were cultivated in Luria Bertani (LB) broth with aeration at 37 °C. L. rhamnosus YT, preserved by the key laboratory of dairy biotechnology and safety control of Jiangsu province, was isolated from the feces of Bama longevity, Guangxi province, China. L. rhamnosus YT was inoculated in dMRS broth at 37 °C in static condition. The biomass was determined by viable counts [39 (link)].
Salmonella enterica wx29
Manufactured by China Center of Industrial Culture Collection
Sourced in China
Salmonella enterica WX29 is a bacterial strain that belongs to the Salmonella enterica species. It is a Gram-negative, rod-shaped bacterium commonly found in the environment and can colonize the gastrointestinal tract of various organisms, including humans and animals. This strain is available as a laboratory resource for research and testing purposes.
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Lab products found in correlation
2 protocols using salmonella enterica wx29
1
Microbial Cultivation and Biomass Determination
2
Probiotic Strains Inhibition Assay
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The LAB strains of Lactobacillus rhamnosus grx19, L. acidophilus ss, L. plantarum s7, L. casei g9, Bifidobacterium animalis BLC and Streptococcus thermophiles 90–57 were isolated from the gut of subjects from Bama longevity, Guangxi province, China, in 2013. The strains were separately cultured in skim milk broth for 12 h at 37 °C. The bacteria and supernatant were separated by centrifugation. The harvested bacteria were suspended by saline to 1 × 108 CFU mL−1. The supernatant was filtered by 0.22 μm Millipore filtration for the assay.
Escherichia coli CICC10899, Salmonella enterica WX29, Staphylococcus aureus CICC10201, Pseudomonas brenneri CICC10271, Clostridium difficile CICC22951, Bacillus subtilis CICC10012 were bought from China Center of Industrial Culture Collection (Beijing, China). All the strains were cultivated in Luria–Bertani broth at 37 °C except the Clostridium difficile CICC22951 cultivated in Reinforced Clostridium Medium broth. The bacteria were harvested after incubation for12 h and suspended to 1 × 107 CFU mL−1 by saline.
Escherichia coli CICC10899, Salmonella enterica WX29, Staphylococcus aureus CICC10201, Pseudomonas brenneri CICC10271, Clostridium difficile CICC22951, Bacillus subtilis CICC10012 were bought from China Center of Industrial Culture Collection (Beijing, China). All the strains were cultivated in Luria–Bertani broth at 37 °C except the Clostridium difficile CICC22951 cultivated in Reinforced Clostridium Medium broth. The bacteria were harvested after incubation for12 h and suspended to 1 × 107 CFU mL−1 by saline.
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