Adipose tissue was immediately collected after terminations and fixed in 10% PBS-buffered formalin for 24 h. Following paraffin embedding, the tissue sections were stained with a primary antibody against Endomucin (1:200, R&D systems. Minneapolis, MN) or Mac-2 (1 mg/ml, 1:1,000; Tebu-Bio, France) followed by donkey α-goat Alexa Fluor 647-conjugated secondary antibodies (1.5 μg/mL, Jackson Immuno-Research Laboratories, West Grove, PA). Nuclei were stained with DAPI (1 μg/mL, Vector Laboratories, Burlingame, CA). Images were acquired with Carl Zeiss upright Apotome Axio Imager Z1/ZEN2 Core Imaging software.
Upright apotome axio imager z1 zen2 core imaging software
Manufactured by Zeiss
The Upright Apotome Axio Imager Z1/ZEN2 Core Imaging software is a lab equipment product from Zeiss. It is a microscope system designed for optical sectioning and structured illumination. The software provides the core functionality to operate the Axio Imager Z1 microscope.
Automatically generated - may contain errors
2 protocols using upright apotome axio imager z1 zen2 core imaging software
1
Adipose Tissue Immunofluorescence Staining
2
Adipose Tissue Immunofluorescence Staining
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Adipose tissue was immediately collected after terminations and fixed in 10% PBS-buffered formalin for 24 h. Following paraffin embedding, the tissue sections were stained with a primary antibody against Endomucin (1:200, R&D systems. Minneapolis, MN) or Mac-2 (1 mg/ml, 1:1,000; Tebu-Bio, France) followed by donkey α-goat Alexa Fluor 647-conjugated secondary antibodies (1.5 μg/mL, Jackson Immuno-Research Laboratories, West Grove, PA). Nuclei were stained with DAPI (1 μg/mL, Vector Laboratories, Burlingame, CA). Images were acquired with Carl Zeiss upright Apotome Axio Imager Z1/ZEN2 Core Imaging software.
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