Q exactive hf biopharma mass spectrometer

Manufactured by Thermo Fisher Scientific

The Q Exactive HF BioPharma mass spectrometer is a high-resolution, accurate-mass (HRAM) instrument that provides sensitive and robust performance for biopharma applications. It features a quadrupole-Orbitrap hybrid design, enabling high-resolution MS and MS/MS analysis.

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Lab products found in correlation

Ultimate 3000 rslcnano, by Thermo Fisher Scientific (1 mentions) Easy nlc 1200, by Thermo Fisher Scientific (1 mentions) Easy spray pepmap c18 column, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Q Exactive HF mass spectrometer Q Exactive HF mass Q Exactive HF spectrometer Q Exactive mass spectrometer Q Exactive HF Exactive mass spectrometer Q Exactive

2 protocols using q exactive hf biopharma mass spectrometer

Peptide Isolation from Liver Tissue

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The iST sample preparation kit (PreOmics, Germany) was used to isolate peptides from liver tissue. Purified peptides were re-dissolved in 20 μl loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and the peptide concentration was determined on a Lunatic instrument (Unchained Lab). 2μg peptides were injected for LC-MS/MS analysis on an Ultimate 3000 RSLCnano system in-line connected to a Q Exactive HF BioPharma mass spectrometer (Thermo). Detailed proteomics methods and analysis are in the supplemental methods. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD023848.

Broadfield L.A., Duarte J.A., Schmieder R., Broekaert D., Veys K., Planque M., Vriens K., Karasawa Y., Napolitano F., Fujita S., Fujii M., Eto M., Holvoet B., Vangoitsenhoven R., Fernandez-Garcia J., Van Elsen J., Dehairs J., Zeng J., Dooley J., Rubio R.A., van Pelt J., Grünewald T.G., Liston A., Mathieu C., Deroose C.M., Swinnen J.V., Lambrechts D., di Bernardo D., Kuroda S., De Bock K, & Fendt S.M. (2021). Fat induces glucose metabolism in non-transformed liver cells and promotes liver tumorigenesis. Cancer research, 81(8), 1988-2001.

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Peptide Analysis by LC-MS/MS

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The peptide samples were analyzed by LC/MS/MS using an Easy nLC 1200 coupled to a QExactive HF Biopharma mass spectrometer (Thermo Fisher Scientific). Samples were injected onto an Easy Spray PepMap C18 column (75 μm id×25 cm, 2 μm particle size) (Thermo Fisher Scientific) and separated over a 2 h method. The gradient for separation consisted of 5-45% mobile phase B at a 250 nl/min flow rate, where mobile phase A was 0.1% formic acid in water and mobile phase B consisted of 0.1% formic acid in acetonitrile (ACN). The QExactive HF was operated in data-dependent mode where the 15 most intense precursors were selected for subsequent fragmentation. Resolution for the precursor scan (m/z 350-1700) was set to 60,000, while MS/MS scans resolution was set to 15,000. The normalized collision energy was set to 27% for HCD. Peptide match was set to preferred, and precursors with unknown charge or a charge state of 1 and ≥7 were excluded.

Cherney R.E., Mills C.A., Herring L.E., Braceros A.K, & Calabrese J.M. (2023). A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB. Biology Open, 12(6), bio059955.

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