Immunohistochemical staining was done on formalin-fixed and paraffin embedded tissue sections from tumors tissue. The tissue was placed in the incubator at 65 °C for 2 h before removing paraffin by xylene. After gradient elute the tissue by absolute ethyl alcohol, 95% ethyl alcohol, 85% ethyl alcohol, washed the tissue by redistilled water. In the third step, target retrieval was achieved with BOND Novocastra Epitope Retrieval Solution 1 (Leica Biosystems) at 100 °C for 20 min. Monoclonal Anti-ki67 antibody (2 µg/ml, Abcam) were used as primary antibody. After that, secondary antibody horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5000, Abcam) were used. In this way, ki67 was stained in the tissue and the tissue was photographed by optical microscope.
Bond novocastra epitope retrieval solution 1
Manufactured by Leica
The BOND Novocastra Epitope Retrieval Solution 1 is a laboratory reagent designed for use in immunohistochemistry and in situ hybridization procedures. Its core function is to facilitate the retrieval and exposure of target epitopes within tissue samples, enabling effective antibody binding and subsequent detection.
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2 protocols using bond novocastra epitope retrieval solution 1
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Immunohistochemical Analysis of Ki67 in Tumor Tissue
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Immunohistochemical Staining of Ki67 in Tumor Tissue
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Immunohistochemical staining was done on formalin-fixed and paraffin embedded tissue sections from tumors tissue. The tissue was placed in the incubator at 65 °C for 2 hours before removing paraffin by xylene. After gradient elute the tissue by absolute ethyl alcohol, 95 % ethyl alcohol, 85 % ethyl alcohol, washed the tissue by redistilled water. In the third step, target retrieval was achieved with BOND Novocastra Epitope Retrieval Solution 1 (Leica Biosystems) at 100 °C for 20 min. Monoclonal Anti-ki67 antibody (2 µg/ml, Abcam) were used as primary antibody. After that, secondary antibody horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5000, Abcam) were used. In this way, ki67 was stained in the tissue and the tissue was photographed by optical microscope.
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