The m-PEA was obtained from Wuxi Cima Science (Wuxi, China). Hematoxylin-Eosin (HE) staining kit was purchased from Solarbio Life Sciences (Beijing, China). Oil Red O was from Sigma-Aldrich (Shanghai, China). Tween-80 and PEG-400 were purchased from Sangon Biotech (Shanghai, China). Anti-PPAR-α antibody (#ab24509) and anti-CD68 antibody (#ab125212) were obtained from Abcam (Shanghai, China). Anti-caspase-1 antibody (clone 14F468) was purchased from Santa Cruz (Shanghai, China). Anti-GAPDH antibody (clone 1E6D9) was purchased from Proteintech (Wuhan, China). Anti-NLRP3 antibody (#A5652) was acquired from ABclonal Technology (Wuhan, China). Anti-α-SMA antibody (clone 1A4) and anti-LC3B antibody (L7543) were obtained from Sigma-Aldrich (Shanghai, China). Antibodies against p62 (#A19700), Beclin1 (#A11761), and ATG7 (#A0691) were obtained from ABconal (Wuhan, China). Horseradish peroxidase (HRP)-conjugated goat anti-mouse and goat anti-rabbit immunoglobulin G (IgG) were purchased from Proteintech (Wuhan, China).
Anti α sma antibody clone 1a4
Manufactured by Merck Group
Sourced in China
The Anti-α-SMA antibody (clone 1A4) is a laboratory reagent produced by Merck Group. It is designed to detect the alpha-smooth muscle actin (α-SMA) protein, which is commonly used as a marker for the identification of myofibroblasts and other cell types in various tissues and cell culture models.
Automatically generated - may contain errors
Lab products found in correlation
Anti lc3b antibody l7543, by Merck Group (1 mentions)
Anti cd68 antibody, by Abcam (1 mentions)
Goat anti rabbit immunoglobulin g igg, by Proteintech (1 mentions)
Hematoxylin eosin staining kit, by Solarbio (1 mentions)
Oil red o, by Merck Group (1 mentions)
Peg 400, by Sangon (1 mentions)
Anti pparα antibody, by Abcam (1 mentions)
Anti caspase1 antibody clone 14f468, by Santa Cruz Biotechnology (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Taqman probe primers sets, by Thermo Fisher Scientific (1 mentions)
Transcriptor first strand cdna synthesis kit, by Roche (1 mentions)
Hoechst 33342, by Dojindo Laboratories (1 mentions)
2 protocols using anti α sma antibody clone 1a4
1
Molecular Mechanism of m-PEA in NAFLD
2
Evaluating α‐SMA Expression in E14C12 Cells
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E14C12 cells were cultured in medium with or without NA808 for 7 days. α‐SMA was immunostained using anti‐α‐SMA antibody clone 1A4 (Sigma‐Aldrich, St. Louis, MO) and the secondary antibody, Goat anti‐mouse IgG antibody Cy3‐conjugated. The fluorescence intensity of Cy3 was measured. Hoechst 33342 (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) fluorescence intensity was measured to detect cell number change. RNA was extracted according to the protocol with a RNeasy Mini Kit (QIAGEN, Venlo, NLD). cDNA samples were synthesized from RNA samples using a Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Science, Penzberg, Germany). Gene expression levels were measured by real‐time PCR using TaqMan probe/primers sets (Applied Biosystems, Waltham, MA, USA) for each gene. The expression level of each gene was corrected for the expression level of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH).
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