In a typical experiment, catalyst powder (25 mg) was suspended in an aniline solution (10 ml, 0.5 mм, Sigma-Aldrich, ≥ 99.5%) in DMSO in the airtight glass reaction tube. The resulting suspension was purged with O2 (5 min, 50 ml min−1) to ensure the reaction mixture was saturated with oxygen. The reaction vessel was subsequently illuminated with solely visible light using the same light source and filter as described above. During the reaction, 0.5 ml aliquots were taken at different time intervals and centrifuged for five minutes at 14,800 rpm to completely separate the catalyst. The resulting supernatant was analyzed by UV-VIS spectroscopy.
Aniline solution
Manufactured by Merck Group
Aniline solution is a clear, colorless to pale yellow liquid chemical compound. It serves as a precursor for the production of various pharmaceutical and industrial products. The solution contains aniline, a primary aromatic amine, as the core ingredient. Aniline solution is commonly used in chemical synthesis and as a laboratory reagent.
Automatically generated - may contain errors
Lab products found in correlation
Polylysine slides, by Thermo Fisher Scientific (1 mentions)
Aperio, by Leica Biosystems (1 mentions)
Phosphomolybdic phosphotungstic acid solution, by Merck Group (1 mentions)
Biebrich scarlet acid fuchsine, by Merck Group (1 mentions)
Neutral buffered formalin, by Merck Group (1 mentions)
Prussian blue, by Merck Group (1 mentions)
Mayer s hematoxylin, by Merck Group (1 mentions)
Weigerts hematoxylin, by Carl Roth (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
Entellan, by Merck Group (1 mentions)
Acetic acid, by Carl Roth (1 mentions)
2 protocols using aniline solution
1
Visible Light-Driven Aniline Oxidation
2
Histological Staining Techniques for Tissue Analysis
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Tissues were fixed for 24 h in 10% neutral buffered formalin (Sigma), dehydrated in ethanol gradients, paraffin embedded and sectioned at 3 μm on polylysine slides (Thermo scientific). Slides were rehydrated with decreasing percentage of ethanol solutions and subjected to stains.
For iron staining, slides were quenched with 3% H2O2 and treated for 20 min with Prussian Blue (Sigma Aldrich) followed by a 20 min incubation with 3,3′-diaminobenzidine (DAB) (Sigma Aldrich). Meyer's hematoxylin was used as counterstain.
For Masson's trichrome staining, slides were treated overnight with the Bourin's mordent solution (Roth). After washing, slides were treated for 5 min with Weigert's hematoxylin (Roth), 5 min with Biebrich Scarlet-Acid Fuchsine (Sigma Aldrich), 5 min with Phosphotungstic/Phosphomolybdic Acid Solution (Sigma Aldrich), 5 min with Aniline Solution (Sigma Aldrich) and 2 min with 1% acetic acid (Carl Roth).
For hematoxylin and eosin staining, slides were treated for 6 min with Mayer's Hematoxylin (Sigma Aldrich), rinsed in phosphate buffered saline, and incubated for 2 min in Eosin in acetic acid (Sigma Aldrich).
After the stains, slides were dehydrated with ethanol gradients and mounted with Entellan (Sigma Aldrich). Digital images were acquired up to 40 × magnification with an automated slide scanner (Aperio - Leica biosystems)
For iron staining, slides were quenched with 3% H2O2 and treated for 20 min with Prussian Blue (Sigma Aldrich) followed by a 20 min incubation with 3,3′-diaminobenzidine (DAB) (Sigma Aldrich). Meyer's hematoxylin was used as counterstain.
For Masson's trichrome staining, slides were treated overnight with the Bourin's mordent solution (Roth). After washing, slides were treated for 5 min with Weigert's hematoxylin (Roth), 5 min with Biebrich Scarlet-Acid Fuchsine (Sigma Aldrich), 5 min with Phosphotungstic/Phosphomolybdic Acid Solution (Sigma Aldrich), 5 min with Aniline Solution (Sigma Aldrich) and 2 min with 1% acetic acid (Carl Roth).
For hematoxylin and eosin staining, slides were treated for 6 min with Mayer's Hematoxylin (Sigma Aldrich), rinsed in phosphate buffered saline, and incubated for 2 min in Eosin in acetic acid (Sigma Aldrich).
After the stains, slides were dehydrated with ethanol gradients and mounted with Entellan (Sigma Aldrich). Digital images were acquired up to 40 × magnification with an automated slide scanner (Aperio - Leica biosystems)
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