Eight arm peg oh 20 kda

Poly-L-lysine hydrobromide (PLL, 120 kDa) was purchased from Polysciences Inc. Heparin sodium (Hep, 16.3 kDa) and chitosan oligosaccharide (ChO) were purchased from Celsus Laboratories and TCI America, respectively. Norbornene-2-carboxylic acid and diethyl ether were purchased from Sigma-Aldrich. Eight-arm PEG-OH (20 kDa) was purchased from JenKem Technology, USA. Derivatives of tetrazine were purchased from Click Chemistry Tools. Lipopolysaccharide (LPS) was purchased from Cell Signaling Technology. Saponin was purchased from Chem-Impex International Inc. 4”,6-Diamidino-2-phenylindole, dihydrochloride (DAPI) was purchased from Anaspec Inc. Sulfo-Cy5-tetrazine was purchased from Santa Cruz Biotechnology. Lysozyme (purified, salt free) was obtained from Worthington Biochemical Corporation. All other chemicals were purchased from Fisher Scientific.

Eight-arm PEG-OH (20 kDa) was acquired from JenKem Technology, USA. 5-Norbornene-2-carboxylic acid, N,N-dicyclohexylcarbodiimide (DCC), 4-(dimethylamino)pyridine (DMAP), dimethyl phenylphosphonite, 2,4,6-trimethylbenzoyl chloride, 2-butanone, lithium bromide, diethyl ether, and Isopropyl-D-1 thogalactopyranoside (IPTG) were obtained from Sigma-Aldrich. Fmoc-protected amino acids N,N,N,N -tetramethyl-O-(1H-benzotriazol-1-yl)uranium hexafluorphosphate (HBTU), and hydroxybenzotriazole (HOBt) were purchased from Anaspec. BL21 Escherichia coli (E. coli) was purchased from New England Biolabs. Kanamycin sulfate was obtained from IBI Scientific. Lysogeny broth, Lennox formulation, agar and broth were obtained from DOT Scientific. Gemcitabine was obtained from TSZ CHEM. Mammalian cell Live/Dead staining kit was obtained from Life Technologies Corp. All other chemicals were obtained from Fisher Scientific unless otherwise noted.