Guanidine hydrochloride

Actual samples including throat swabs collected from the patients diagnosed with Mycoplasma pneumoniae infection, human papilloma virus type 16 (HPV-16) positive cervical swabs, as well as gastric mucosa of the patients suffering severe stomachache with suspected Helicobacter pylori infection were provided by the Affiliated Hospital of Qingdao University. All the samples were immediately stored at −20 °C after collection for subsequently use. Streptococcus aureus (ATCC 25923), Salmonella typhimurium strains (ATCC 14028) were provided by Navid Biotechnology Co., Ltd (Qingdao, China). SARS-CoV-2 pseudovirus was purchased from Fubio Biological Technology Co., Ltd (Shanghai, China). Guanidine hydrochloride, guanidine isothiocyanate, proteinase K, ethanol, isopropanol, SDS, TritonX-100, Tween-20 and pig serum were purchased from Sangon Biotech Co., Ltd (Shanghai, China). All the other chemicals and reagents were of analytical grade.

Bst 2.0 WarmStart DNA polymerase, MgSO₄ (100 mM) and 10 × Thermopol Isothermal buffer containing 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH4)₂SO₄, 20 mM MgSO₄ and 1 % Tween-20 were purchased from New England BioLab (NEB, USA). A 10 mM deoxynucleotide (dNTP) solution was purchased from Vazyme Biotech (China). Eva Green dye was purchased from Biotium Inc. (USA). SYBR Green dye was purchased from Solarbio Science & Technology (China). Tween-20, Triton X-100, dithiothreitol and guanidine hydrochloride were purchased from Sangon Biotech (China).