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Anti p t446 pkr antibody

Manufactured by Abcam
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Anti-p-T446 PKR antibody is a primary antibody that detects phosphorylation of threonine 446 in the protein kinase R (PKR) enzyme. This antibody can be used in various applications to analyze the phosphorylation status of PKR.

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Lab products found in correlation

Anti flag antibody f1804, by Merck Group (2 mentions) Anti cdk1 antibody, by Cell Signaling Technology (2 mentions) Anti cyclin b1 sc 245, by Santa Cruz Biotechnology (2 mentions) Anti pkr monoclonal antibody, by Santa Cruz Biotechnology (2 mentions) Anti cdc27 sc 9972, by Santa Cruz Biotechnology (2 mentions) Anti β actin sc 47778, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Anti-PKR (9 citations) P-PKR (5 citations) Anti-PKR-p (T446; (2 citations)

2 protocols using anti p t446 pkr antibody

1

Phospho-specific antibodies against PKR

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-PKR monoclonal antibody from Santa Cruz Biotechnology (sc-6282, 1:1000) and Cell Signaling Technology (12297, 1:2000) was used throughout the study. The anti-p-T446 PKR antibody was from Abcam (ab32036, 1:1000). Anti-CDK1 antibody (9116, 1:1000) was from Cell Signaling Technology. Anti-cyclin B1 (sc-245, 1:2000), anti-CDC27 (sc-9972, 1:1000), anti-GFP (sc-9996, 1:2000), and anti-β-actin (sc-47778, 1:2000) antibodies were obtained from Santa Cruz Biotechnology. Anti-Flag antibody (F1804, 1:2000) was from Sigma-Aldrich. Rabbit polyclonal phospho-specific antibodies against PKR S83, S456, and S542 were generated and purified by AbMart, Inc. The peptides used for immunizing rabbits were EKKAV-pS-PLLLT (S83), TLRYM-pS-PEQIS (S456), and TVWKK-pS-PEKNE (S542). The corresponding non-phosphorylated peptides were also synthesized and used for antibody purification and blocking assays. Other antibodies used in this study were provided in the supplemental table.
Yin L., Zeng Y., Zeng R., Chen Y., Wang T.L., Rodabaugh K.J., Yu F., Natarajan A., Karpf A.R, & Dong J. (2021). Protein Kinase RNA-activated Controls Mitotic Progression and Determines Paclitaxel Chemosensitivity through B-cell lymphoma 2 in Ovarian Cancer. Oncogene, 40(50), 6772-6785.
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2

Phospho-specific antibodies against PKR

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-PKR monoclonal antibody from Santa Cruz Biotechnology (sc-6282, 1:1000) and Cell Signaling Technology (12297, 1:2000) was used throughout the study. The anti-p-T446 PKR antibody was from Abcam (ab32036, 1:1000). Anti-CDK1 antibody (9116, 1:1000) was from Cell Signaling Technology. Anti-cyclin B1 (sc-245, 1:2000), anti-CDC27 (sc-9972, 1:1000), anti-GFP (sc-9996, 1:2000), and anti-β-actin (sc-47778, 1:2000) antibodies were obtained from Santa Cruz Biotechnology. Anti-Flag antibody (F1804, 1:2000) was from Sigma-Aldrich. Rabbit polyclonal phospho-specific antibodies against PKR S83, S456, and S542 were generated and purified by AbMart, Inc. The peptides used for immunizing rabbits were EKKAV-pS-PLLLT (S83), TLRYM-pS-PEQIS (S456), and TVWKK-pS-PEKNE (S542). The corresponding non-phosphorylated peptides were also synthesized and used for antibody purification and blocking assays. Other antibodies used in this study were provided in the supplemental table.
Yin L., Zeng Y., Zeng R., Chen Y., Wang T.L., Rodabaugh K.J., Yu F., Natarajan A., Karpf A.R, & Dong J. (2021). Protein Kinase RNA-activated Controls Mitotic Progression and Determines Paclitaxel Chemosensitivity through B-cell lymphoma 2 in Ovarian Cancer. Oncogene, 40(50), 6772-6785.
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