The largest database of trusted experimental protocols

2 protocols using dichlorofluorescein dcf diacetate

1

Mitochondrial Dysfunction Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
3,4-dihydroxyphenylacetic acid, quercetin, sulforaphane hemin, rhodamine123 (rhod), dichlorofluorescein (DCF) diacetate, triphenyltin,1,1,3,3-Tetramethoxypropane, 2,6-dichlorophenolin-dophenol (DCIP), rotenone, NADH, succinate, malonate and puromycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). CellTiter96® AQueous Assay (Madison, WI, USA) was obtained from Promega (Madison, Wi, USA) and DNA/RNA oxidation ELISA kit from Cayman Chemicals (Ann Arbour, MI, USA). Pierce BCA Protein Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). MitoSoxTM Red mitochondrial was obtained from Molecular Probes (Eugene, OR, USA).
+ Open protocol
+ Expand
2

Tanshinone IIA Modulates Renal Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pentobarbital sodium salt and dichloro-fluorescein (DCF) diacetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tanshinone IIA (Tan IIA) was obtained from Zhejiang Institute for Drug Control (Hangzhou, Zhejing, China). G1 and G15 were purchased from ApexBio (Houston, TX, USA). The SOD assay kit was purchased from NanJin Jin Cheng Bioengineering Institute (NanJing, Jingshu, China). Annexin V−/PI staining kit was purchased from Yeasen Biotech (Pudong, Shanghai, China). The phospho-MEKSer221, phospho-ERK1/2Thr202 and phospho-GSK-3βSer9 antibodies were purchased from OmnimAbs (Alhambra, CA, USA), and the cleaved caspase-3 antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). The Tamm-Horsfall protein, aquaporin-1 and nephrin were purchased from Affinity Biosciences (Cincinnati, OH, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!