Sab5700724

Manufactured by Merck Group

Sourced in United States

The SAB5700724 is a laboratory equipment product manufactured by the Merck Group. It is designed for specialized scientific applications. The core function of this product is to perform specific tasks in a laboratory setting. However, a detailed description of its intended use cannot be provided in an unbiased and factual manner without the risk of extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Xtractor buffer kit, by Takara Bio (2 mentions) Hrp conjugated anti rabbit igg secondary antibody solution, by Affinity Biosciences (2 mentions) Ecl kit, by GLPBIO (2 mentions) Ab76011, by Abcam (2 mentions) Ab52235, by Abcam (2 mentions) Ab32034, by Abcam (2 mentions) S0001, by Affinity Biosciences (2 mentions) Af7018, by Affinity Biosciences (2 mentions) C6198, by Merck Group (1 mentions) S1700, by Agilent Technologies (1 mentions) Ab32503, by Abcam (1 mentions) Bca assay kit, by Beyotime (1 mentions) Ab196495, by Abcam (1 mentions)

3 protocols using sab5700724

Immunohistochemical Analysis of Fibrosis Markers

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Four-micrometer sections of formalin-fixed and paraffin-embedded specimens were deparaffinated, followed by antigen retrieval using Target Retrieval (S1700, Dako). The sections were subsequently blocked with phosphate-buffered saline plus 10% donkey serum and 3% bovine serum albumin and stained with anti–ACTA2-Cy3(1:400; C6198, Sigma-Aldrich), anti-collagen 1 alpha 1 (COL1A1) (1:200; ab279711, Abcam), anti-fibronectin 1 (FN1) (1:200; SAB5700724, Sigma-Aldrich), anti-pSMAD3 (0.5 μg/ml; LS-B64-50, LifeSpan BioSciences), anti-TAZ (1:400; ZRB1260, Sigma-Aldrich), anti-YAP (1:200; 4912, Cell Signaling Technology), anti-Ki67 (1:200; 14-5698-82, eBioscience), or anti-γH2AX (1:200; MAB15111, Abnova).

Ma H.Y., Li Q., Wong W.R., N’Diaye E.N., Caplazi P., Bender H., Huang Z., Arlantico A., Jeet S., Wong A., Emson C., Brightbill H., Tam L., Newman R., Roose-Girma M., Sandoval W, & Ding N. (2023). LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lung. Science Advances, 9(21), eadf0133.

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Western Blot Analysis of SV40-MES-13 Cells

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SV40-MES-13 cells were lysed with the xTractor Buffer Kit (Clontech Laboratories, Inc., Mountain View, CA, USA). Protein specimens were quantified through the BCA assay kit (Beyotime, Shanghai, China) and electrophoresed through 10% SDS-PAGE gels. After transferring onto PVDF membranes, interested proteins were masked with 2% non-fat milk at 37 °C for 30 min, then incubated with primary antibodies against FN (1:700, SAB5700724; Sigma-Aldrich, St. Louis, MO, USA), Col-IV (1:500, ab52235; Abcam, Fremont, CA, USA), CDKN1B (1:1,000, ab32034; Abcam, Fremont, CA, USA), N-cad (1:8,000, ab76011; Abcam, Fremont, CA, USA), Bax (1:3,000, ab32503; Abcam, Fremont, CA, USA), Bcl-2 (1:1,500, ab196495; Abcam, Fremont, CA, USA), and β-actin (1:20,000, AF7018; Affinity, Jiangsu, China) at 4 °C for 20 h. The membranes were rinsed three times with PBST, then immersed in HRP-conjugated anti-rabbit IgG secondary antibody solution (1:5,000, S0001; Affinity, Jiangsu, China) at 37 °C for 60 min. Interesting proteins were visualized using a commercial ECL kit (Glpbio, Montclair, CA, USA). Fluorescence intensities were quantified using Fiji software.

Sun L., Ding M., Chen F., Zhu D, & Xie X. (2023). Long non‑coding RNA L13Rik promotes high glucose-induced mesangial cell hypertrophy and matrix protein expression by regulating miR-2861/CDKN1B axis. PeerJ, 11, e16170.

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Western Blot Analysis of Mesangial Cells

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Mesangial cells were lysed with xTractor Buffer Kit (CLONTECH Laboratories, Inc., CA, USA). Protein specimens were quantified through BCA method and electrophoresed through 10% SDS-PAGE gels. After transferring onto PVDF membranes, interested proteins were masked with 2% non-fat milk at 37 ℃ for 30 min, then incubated with anti-FN antibody (1:700; SAB5700724; Sigma-Aldrich), anti-Col IV antibody (1: 500; ab52235; Abcam, CA, USA), anti-CDKN1B antibody (1: 1000; ab32034; Abcam), anti-N-cad antibody (1:8000; ab76011; Abcam), and anti-β-actin antibody (1:20000; AF7018; Affinity, Jiangsu, China) at 4 ℃ for 20 h. The membranes were rinsed 3 times with PBST, then immersed in HRP-conjugated anti-rabbit IgG secondary antibody solution (1: 5000; S0001; Affinity) at 37 ℃ for 60 min. Interesting proteins were visualized using commercial ECL kit (Glpbio, CA, USA). Fluorescence intensities were quantified using Fiji software.

Sun L., Ding M., Chen F., Zhu D., Xie X., & Wang X. (2022). Long non-coding RNA L13Rik promotes high glucose-induced mesangial cell hypertrophy and matrix protein expression by regulating miR-2861/CDKN1B axis.

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