Tumor and lymph node cells isolated from mice were stained with LIVE/DEAD Fixable Violet Dead Staining Kit (Invitrogen) and labeled with a cocktail of monoclonal antibodies conjugated with fluorochromes: Anti-CD45 (30-F11) BV 605, anti-CD3 (17A2) BV 650, anti-CD4 (RM4-5) FITC, anti-CD8 (53–6.7) APC/Fire750, anti-CD25 (PC61) PE, anti-CD44 (IM7) PE-Cy7, anti-CD62L (MEL-14) PerCP-Cy5.5 (all from BioLegend), and anti-CD49b (DX5) PE-CF594 (BD Biosciences) for lymphoid cell analysis in tumors and anti-CD4 (RM4-5) PerCP-Cy5.5, anti-CD8 (53-6.7) PE-Cy, anti-CD44 (IM7) FITC, and anti-CD62L (MEL-14) BV 605 (all from BioLegend) for lymphocyte analysis in tLNs. In the following step, the cells were fixed using eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen). Subsequently, cells from tLNs were labeled with anti-Ki67 (16A8) APC (BioLegend). Analyses of lymphoid cells in tumors and tLNs were performed using LSRFortessa (BD Biosciences).
Anti cd49b dx5 pe cf594
Manufactured by BD
Anti-CD49b (DX5) PE-CF594 is a fluorescently-labeled monoclonal antibody that binds to the CD49b cell surface marker. CD49b is expressed on natural killer (NK) cells, a type of lymphocyte involved in the innate immune response. This reagent can be used to identify and enumerate NK cells in flow cytometry experiments.
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Lab products found in correlation
Lsrfortessa, by BD (2 mentions)
Ebioscience foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)
Anti cd4 rm4 5 percp cy5, by BioLegend (1 mentions)
Anti cd8 53 6.7 pe cy, by BioLegend (1 mentions)
Anti cd11b m1 70 percp cy5, by BioLegend (1 mentions)
Anti f4 80 bm8 alexa fluor 700, by BioLegend (1 mentions)
Anti ly6g 1a8 bv 605, by BioLegend (1 mentions)
2 protocols using anti cd49b dx5 pe cf594
1
Multiparametric Flow Cytometry of Tumor-Infiltrating Lymphocytes
2
Characterizing Tumor-Infiltrating Myeloid Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tumor cells were isolated from mice were labeled with a cocktail of monoclonal antibodies conjugated with fluorochromes: Anti-CD45 (30-F11) V500, anti-CD3 (145-2C11) PE-CF594, anti-CD19 (1D3) PE-CF594, anti-CD49b (DX5) PE-CF594 (all from BD Biosciences), anti-CD11b (M1/70) PerCP-Cy5.5, anti-CD11c (N418) BV 650, anti-F4/80 (BM8) Alexa Fluor 700, anti-Ly6C (HK1.4) PE, anti-Ly6G (1A8) BV 605 (all from BioLegend). In order to exclude dead cells, DAPI dye was added prior to the analysis. Analysis of percentage of tumor-infiltrating myeloid cells as well as EGFP expression was performed using the LSRFortessa (BD Biosciences).
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