Moflo astrios eq device

Cells were gently detached using trypsin and rinsed in PBS. After removal of cell clumps (70 μm cell strainer), individual cells were resuspended into 500 μL of PBS-0.1% BSA. The cells were analyzed on a cytoFlex device (Beckman Coulter) and data were analyzed with the CytoExpert software (Beckman Coulter). For FACS, infected neurons were sorted using a MoFlo Astrios EQ device (Beckman Coulter). Negative cells (either not transfected or transduced) were used to set up the gates.

Cells were gently detached using trypsin and rinsed in PBS. After removal of cell clumps (70 μm cell strainer), individual cells were resuspended into 500 μL of PBS-0.1% BSA. The cells were analyzed on a cytoFlex device (Beckman Coulter) and data were analyzed with the CytoExpert software (Beckman Coulter). For FACS, infected neurons were sorted using a MoFlo Astrios EQ device (Beckman Coulter). Negative cells (either not transfected or transduced) were used to set up the gates.

To purify intestinal ILCs for analysis in cell culture or for RNASeq analysis, we performed fluorescence activated cell sorting using a MoFlo Astrios EQ device (Beckman Coulter) within the Core Unit Cell Sorting Erlangen. ILCs were purified using the following surface marker panel: CD45⁺ (REA737 in VioBlue, 1:50), B220^- (REA755 in FITC, 1;50), CD3^- (REA641 in FITC, 1:50), CD5^- (in FITC, 1:50), CD11b^- (REA592 in APC-Vio770, 1:50), CD11c^- (REA754 in APC-Vio770, 1:50), KLRG1^- (2F1 in APC; eBioscience, 1:158), CD127⁺ (IL-7Rα; A7R34 in PE; BioLegend, 1:20), Thy1.2⁺ (in PerCP-Vio700, 1:20). Unless specified otherwise, all antibodies used for cell sorting were purchased from Miltenyi Biotec.