Blood samples were collected after a 12-h overnight fast and stored at < 80°C until analyzed. An Olympus AU400 automatic analyzer (Olympus Corporation, Tokyo, Japan) was used to measure serum total cholesterol (TC), High Density Lipoprotein cholesterol (HDL-C), triglycerides (TG) and low Density Lipoprotein cholesterol (LDL-C) was calculated. Fasting blood glucose (FBG) was measured with commercial kits (Roche Diagnostics, Indianapolis, IN, USA) and fasting blood insulin (FBI) were determined with the Phadebas Insulin Test (Pharmacia, Uppsala, Sweden) using a radioimmunosorbent technique. The insulin sensitivity was then calculated using Homeostasis Model Assessment (HOMA-IR) according to the following formula: HOMA-IR = FBG (mmol/L) ×FBI (μU/ml)/22.5.
Au400 automatic analyzer
Manufactured by Olympus
Sourced in Japan
The AU400 is an automatic analyzer designed for clinical chemistry testing. It is capable of performing a variety of diagnostic assays, including biochemical, immunochemical, and turbidimetric tests. The AU400 is equipped with a high-throughput system and can process a large number of samples efficiently.
Automatically generated - may contain errors
4 protocols using au400 automatic analyzer
1
Blood Lipid and Insulin Sensitivity Assessment
2
Comprehensive Metabolic Profiling Study
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Fasting blood and urine samples were collected from each participant. The centrifuged serum samples were stored at -20°C. After investigation and collection, all samples were airlifted to the Shenyang Central Laboratory through the cold chain system. Laboratory tests were performed on venous blood samples, which were collected from each subject after an 8-h fast, to measure fasting plasma glucose (FPG), lipid profile, glycosylated hemoglobin (HbA1c). An oral glucose tolerance test (OGTT) was performed in all subjects except those diagnosed with diabetes. We used the hexokinase enzymatic method to measure PG and OGTT–2 h PG (Au400 automatic analyzer, Olympus company, Japan; reagent purchased from Daiichi Pharmaceutical Co. Ltd). Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL–C), high-density lipoprotein cholesterol (HDL–C) and uric acid (UA) were assessed using reagents from Mindray Company (BS-180). Bio-Rad reagents were used for HbA1c measurement.
3
Comprehensive Thyroid and Metabolic Assessment
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After the survey was completed, all specimens collected were airlifted through a cold chain system to the institute of Endocrinology of China Medical University for centralized tests. Blood glucose (BG) was tested by the Hexokinase method (Au400 automatic analyzer, Olympus, Japan). Serum triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were determined by Mindray's kit and biochemical detector (BS-180, Mindray, Shenzhen, China). The glycated hemoglobin (HbA1c) assay was measured by Bio Rad reagents. Serum TSH, TPOAb, and TgAb levels were determined by the electrochemical luminescence immunoassay using the Cobas 601 analyzer (Roche Diagnostic, Switzerland). Urine iodine concentration (UIC) was examined using an inductively coupled plasma mass spectrometry (ICP-MS) (Agilent 7700x, Agilent Technologies, USA).
All participants underwent thyroid ultrasonography by qualified medical staffs using a portable instrument (LOGIQ 100 PRO, GE, Milwaukee, WI, USA with 7.5 MHz linear transducers).
All participants underwent thyroid ultrasonography by qualified medical staffs using a portable instrument (LOGIQ 100 PRO, GE, Milwaukee, WI, USA with 7.5 MHz linear transducers).
4
Metabolic Biomarkers in Fasted Individuals
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Blood samples were collected after a 12-h overnight fast and stored at <80 °C until analyzed. An Olympus AU400 automatic analyzer (Olympus Corporation, Tokyo, Japan) was used to measure serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), triglycerides (TG) and fasting blood glucose(FBG); fasting blood insulin (FBI) were measured with commercial kits (Roche Diagnostics, Indianapolis, IN, USA). Low density lipoprotein cholesterol (LDL-C) was calculated according to certain equation (LDL-C = Total cholesterol − Triglycerides/5 + HDL-C). The insulin sensitivity was then calculated using Homeostasis Model Assessment (HOMA-IR) according to the following formula: HOMA-IR = FBG (mmol/l) × FBI (μU/ml)/22.5.
The cut-off level of HOMA-IR was >3.0 to specify insulin resistance.21 (link)
Serum 25(OH) D levels were measured by immunoassay (DiaSorin 25-OH D assay, Still water, Minnesota, USA). Serum IL-6 and high-sensitive C-reactive protein (hs-CRP) levels were measured by commercially available sandwich enzyme-linked immunosorbent assay ELISA kits.
The cut-off level of HOMA-IR was >3.0 to specify insulin resistance.21 (link)
Serum 25(OH) D levels were measured by immunoassay (DiaSorin 25-OH D assay, Still water, Minnesota, USA). Serum IL-6 and high-sensitive C-reactive protein (hs-CRP) levels were measured by commercially available sandwich enzyme-linked immunosorbent assay ELISA kits.
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