The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Thermo Fisher Scientific, 13-1900, 1 : 100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc., #25-6790, 1 : 200 dilution), anti-Histone H3 (phospho S28) rat polyclonal antibody (Abcam, #ab10543, 1 : 200 dilution), Alexa Fluor 546-conjugated goat anti-rat IgG (H + L) antibody (Thermo Fisher Scientific, #A11081, 1 : 1000 dilution) and Alexa Fluor 647-conjugated goat anti-rabbit IgG (H + L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1 : 1000 dilution). We used MMC (Nacalai Tesque, #20898-2).
Alexa fluor 546 conjugated goat anti rat igg h l antibody
Manufactured by Thermo Fisher Scientific
Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody is a secondary antibody used for detection and visualization in immunoassays. It is conjugated with the Alexa Fluor 546 fluorescent dye, which emits light in the orange-red region of the visible spectrum upon excitation.
Automatically generated - may contain errors
3 protocols using alexa fluor 546 conjugated goat anti rat igg h l antibody
1
Immunofluorescence Staining for E-cadherin, Myosin-VIIa, and Histone H3
2
Immunofluorescence of E-cadherin
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The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Thermo Fisher Scientific, #13-1900, 1:100 dilution) and Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000 dilution).
3
Immunofluorescence Staining of Cell Markers
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The following primary and secondary antibodies were used for immunofluorescence: anti-Ecadherin rat antibody (Cell Signaling Technology, #3195, 1:100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc., #25-6790, 1:200 dilution), anti-Histone H3 (phospho S28) rat polyclonal antibody (Abcam, #ab10543, 1:200 dilution), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000 dilution).
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