Softmax pro 7.1 gxp software

HSV-2–infected or romidepsin-treated cells were fractionated with Minute Cytoplasmic & Nuclear Extraction Kits (SC-003, Invent Biotechnologies Inc.), and at least 0.5 μg of nuclear extract was used to determine HDAC activity (Epigenase HDAC Activity/Inhibition Direct Assay Kit, P-4034-96, EpigenTek). Absorbance was measured using a SpectraMax M5e microplate reader and SoftMax Pro 7.1 GxP software (Molecular Devices).

Media from NP swabs were thawed and treated with UV light to inactivate virus prior to use. SARS-CoV-2–specific IgG and IgA were measured using MILLIPLEX SARS-CoV-2 Antigen Panel 1 IgG and IgA kits (MilliporeSigma, HC19SERG1-85K and HC19SERA1-85K, respectively). Cytokines were measured using the MILLIPLEX Human Cytokine/Chemokine/Growth Factor Panel A kit (MilliporeSigma, HCYTA-60K). Data from Luminex assays were acquired on a Luminex MAGPIX and analyzed in the MILLIPLEX Analyst program (MilliporeSigma). Total IgA was measured using the IgA Human ELISA Kit (Invitrogen, Thermo Fisher Scientific, BMS2096) and total IgG measured using the IgG Human ELISA Kit (Invitrogen, Thermo Fisher Scientific, BMS2091). ELISA data were acquired on a Spectra Max M5 using SoftMax Pro 7.1 GxP software (both Molecular Devices). Total protein was measured by NanoDrop.