Diazonium fast blue rr salt

Manufactured by Merck Group

Diazonium fast blue RR salt is a chemical compound used in various analytical and synthetic applications in laboratory settings. It serves as a versatile reagent for the detection and identification of certain functional groups and compounds. The core function of this product is to provide a colorimetric response when reacting with specific analytes, enabling researchers to perform various chemical analyses and assays.

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Lab products found in correlation

Naphthol as tr phosphate, by Merck Group (3 mentions) L tartaric acid, by Merck Group (2 mentions) N n dimethylformamide (dmf), by Merck Group (2 mentions) Axio 100, by Zeiss (1 mentions) E600pol, by Nikon (1 mentions)

Spelling variants of this product

Fast Blue (39 citations) Fast Blue RR salt (31 citations) Fast Blue RR (16 citations) TM-blue (2 citations)

3 protocols using diazonium fast blue rr salt

Osteoclast and Osteogenic Cell Evaluation

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TRAP was used to evaluate osteoclasts by using 2.5 mM naphthol AS-TR phosphate (Sigma-Aldrich), 0.36 M N-N-dimethyl-formamide (Sigma-Aldrich), and 4 mM salt in pH 5.2 acetate buffer. Nonosteoclastic acid phosphatase activity was inhibited with 100 mM l(+)-tartaric acid (Sigma-Aldrich) added to the substrate solution.
ALP was used to reveal the layer of osteogenic cells (pre-osteo/cementoblasts and osteo/cementoblasts) by incubating the sections with naphthol-ASTR-phosphate (Sigma-Aldrich) and diazonium Fast Blue RR salt (Sigma-Aldrich) for 30 min at 37°C (pH 9) in the presence of MgCl₂.

Zhukouskaya V.V., Jauze L., Charles S., Leborgne C., Hilliquin S., Sadoine J., Slimani L., Baroukh B., van Wittenberghe L., Danièle N., Rajas F., Linglart A., Mingozzi F., Chaussain C., Bardet C, & Ronzitti G. (2021). A novel therapeutic strategy for skeletal disorders: Proof of concept of gene therapy for X-linked hypophosphatemia. Science Advances, 7(44), eabj5018.

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Histological Analysis of Osteogenic Differentiation

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Samples were fixed in 4% paraformaldehyde and embedded in paraffin for sectioning (n = 6 per group). Seven micrometer thick serial sections were dewaxed, rehydrated and stained with von Kossa staining, Sirius Red, processed for alkaline phosphatase (ALP) activity used as a marker of osteogenic cell differentiation. Histology sections were incubated with naphthol ASTR phosphate (Sigma–Aldrich, St. Louis, MO) and diazonium fast blue RR salt (Sigma–Aldrich) for 30 minutes at 37°C (pH 9) in the presence of MgCl₂. Alizarin Red was used as a mineralization marker, showing specifically calcium ions. The slides were rinsed, dehydrated and mounted for observation with an optical microscope (Nikon E600 POL) or an epifluorescence microscope (AXIO 100 Zeiss). Marked cells (ALP) and calcium deposits (Alizarin Red) were quantified using ImageJ software (six sections were counted for each sample).

Novais A., Lesieur J., Sadoine J., Slimani L., Baroukh B., Saubaméa B., Schmitt A., Vital S., Poliard A., Hélary C., Rochefort G.Y., Chaussain C, & Gorin C. (2019). Priming Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth with Fibroblast Growth Factor‐2 Enhances Mineralization Within Tissue‐Engineered Constructs Implanted in Craniofacial Bone Defects. Stem Cells Translational Medicine, 8(8), 844-857.

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Osteoclast and Osteogenic Cell Evaluation

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Tartrate-resistant acid phosphatase (TRAP) was used to evaluate osteoclasts by using 2.5 mM naphthol-ASTR-phosphate (Sigma–Aldrich), 0.36 M N–N-dimethyl-formamide (Sigma–Aldrich), and 4 mM salt in pH 5.2 acetate buffer. Non-osteoclastic acid phosphatase activity was inhibited with 100 mM L(+)-tartaric acid (Sigma–Aldrich) added to the substrate solution.
Alkaline phosphatase was used to reveal the layer of osteogenic cells by incubating the sections with naphthol ASTR phosphate (Sigma–Aldrich) and diazonium fast blue RR salt (Sigma–Aldrich) for 30 min at 37°C (pH 9) in the presence of MgCl₂.

Cauliez A., Zhukouskaya V.V., Hilliquin S., Sadoine J., Slimani L., Miceli-Richard C., Briot K., Linglart A., Chaussain C, & Bardet C. (2021). Impact of Early Conventional Treatment on Adult Bone and Joints in a Murine Model of X-Linked Hypophosphatemia. Frontiers in Cell and Developmental Biology, 8, 591417.

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