Ago2 antibody clone 11a9

Manufactured by Merck Group

Sourced in United States

The AGO2 antibody (clone 11A9) is a laboratory research tool used to detect the presence and distribution of the AGO2 protein in biological samples. AGO2 is a key component of the RNA-induced silencing complex (RISC), which plays a central role in RNA interference (RNAi) pathways. This antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of AGO2 in cells and tissues.

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Lab products found in correlation

Protein g dynabead, by Thermo Fisher Scientific (1 mentions) Pierce crosslink ip kit, by Thermo Fisher Scientific (1 mentions) Uv crosslinker, by Spectroline (1 mentions)

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Ago2) antibody (123 citations)

2 protocols using ago2 antibody clone 11a9

Immunoprecipitation of AGO2 Protein

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Protein G Dynabeads (Life Technologies) were prepared using 100 μl of beads coupled to 10 μg of AGO2 antibody (clone 11A9, Merck) or IgG control antibody (Rat IgG2a kappa Isotype Control (eBR2a), Invitrogen) per sample. Beads were equilibrated in lysis buffer before the lysate was added to the beads and incubated rotating at 4°C overnight. The beads were then washed as followed.
All following reactions included 80 U RNase inhibitors and incubations were carried out with intermittent shaking.

Ressel S., Kumar S., Bermúdez-Barrientos J.R., Gordon K., Lane J., Wu J., Abreu-Goodger C., Schwarze J, & Buck A.H. (2024). RNA–RNA interactions between respiratory syncytial virus and miR-26 and miR-27 are associated with regulation of cell cycle and antiviral immunity. Nucleic Acids Research, 52(9), 4872-4888.

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Identification of miR-106b targets using Ago2-IP

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BxPc-3 cells were mock transfected or transfected with biotinylated has-miR-106b mimic. Forty-eight hours post-transfection, cells were placed on ice, irradiated once with 150 mJ/cm² at 254 nm using a UV Crosslinker (Spectroline, Westbury, NY) and lysed for 15 minutes on ice in lysis buffer. Five mg of of post-nuclear extracts were subjected to immunoprecipitation using 10 μg of Ago2 antibody (clone 11A9, EMD Millipore, Billerica, MA, USA) or 10 μg of rat IgG using Pierce Crosslink IP Kit (Thermo Scientific, Pittsburgh, PA). The rest of the immunoprecipitated complex was digested with 30 μg of proteinase K to release the ribonucleoprotein complex and a second round of immunoprecipitation was performed using anti-streptavidin antibody (EMD Millipore). RNA extraction and qRT-PCR was done as described above. Relative enrichment was calculated from the qRT-PCR data.

Luo Z.L., Luo H.J., Fang C., Cheng L., Huang Z., Dai R., Li K., Tian F.Z., Wang T, & Tang L.J. (2015). Negative correlation of ITCH E3 ubiquitin ligase and miRNA-106b dictates metastatic progression in pancreatic cancer. Oncotarget, 7(2), 1477-1485.

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