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E cadherin sc 21791

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

E-Cadherin (sc-21791) is a lab equipment product offered by Santa Cruz Biotechnology. It is a protein that plays a role in cell-cell adhesion.

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Lab products found in correlation

N cadherin sc 271386, by Santa Cruz Biotechnology (1 mentions) Tris glycine gel, by Bio-Rad (1 mentions) Myecl imager, by Thermo Fisher Scientific (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ab109250, by Abcam (1 mentions) Ab195377, by Abcam (1 mentions) Ab207607, by Abcam (1 mentions) Ab97959, by Abcam (1 mentions) Ab6489, by Abcam (1 mentions) Ecl plus, by Merck Group (1 mentions) Ac026, by Abcam (1 mentions) Ab9485, by Abcam (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Sc 25778, by Santa Cruz Biotechnology (1 mentions) Ecl plus western blotting detection reagent, by GE Healthcare (1 mentions)

3 protocols using e cadherin sc 21791

1

Western Blot Analysis of Cell Signaling

Cited 1 time
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Treated MB cells were washed twice with ice-cold PBS and whole cell extracts (WCE) were prepared by lysing cells in modified RIPA buffer and fractionated in 4–20% Tris-Glycine Gels (Bio-Rad, Hercules, CA). Western Blot analysis were performed by transferring proteins to PVDF membranes (Millipore, Bedford, Mass) as previously described [24 (link), 25 (link)]. Membranes were probed using the appropriate primary and secondary antibodies, diluted according to the manufacturers’ instructions. Signals were detected by enhanced chemiluninescence and using MyECL imager (ThermoScientific, MA, USA).
Primary antibodies (Abs) used for Western blots are as follows:
STAT3 (sc-483 and sc-482), MYC (9E10, sc-40), BCL2 (sc-7382), CCND1 (sc-753), β-Actin (sc-47778), E-Cadherin (sc-21791), N-Cadherin (sc-271386), Vimentin (sc-6260), CHOP (sc-7351), PIAS3 (sc-46682) and OCT4 (sc-5279) were purchased from Santa Cruz (Dallas, TX).
P-Tyr 705 STAT3 (# 9145), PARP (# 9532), GAPDH (# 5174), Vinculin (# 3901), Cyclophilin (# 2175) were purchased from Cell Signaling (Danvers, MA). Nestin Ab (ABD69) was purchased from Millipore (Billerica, MA).
Ray S., Coulter D.W., Gray S.D., Sughroue J.A., Roychoudhury S., McIntyre E.M., Chaturvedi N.K., Bhakat K.K., Joshi S.S., McGuire T.R, & Sharp J.G. (2018). Suppression of STAT3 NH2-Terminal Domain Chemosensitizes Medulloblastoma Cells by Activation of Protein Inhibitor of Activated STAT3 via de-repression by MicroRNA-21.. Molecular carcinogenesis, 57(4), 536-548.
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2

Stem Cell Marker Expression Analysis

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Total cell lysates were collected in modified RIPA lysis buffer. Primary antibodies were used as follows: HIF-1α (ab6489, 1:1000, Abcam, Cambridge, MA, USA), HIF-2α (ab207607, 1:1000, Abcam), ALKBH5 (ab195377, 1:1000, Abcam), SOX2 (ab97959, 1:1000, Abcam), NANOG (ab109250, 1:1000, Abcam), ALDH1 (#36671, 1:1000, CST), E-cadherin (sc-21791, 1:1000, Santa Cruz), GAPDH (ab9485, 1:5000, Abcam) and ACTIN (AC026 1:5000, Abcam). HRP-conjugated anti-rabbit and anti-mouse antibodies were used, and chemiluminescent signals were detected with ECL Plus (Millipore).
Chen G., Liu B., Yin S., Li S., Guo Y., Wang M., Wang K, & Wan X. (2020). Hypoxia induces an endometrial cancer stem-like cell phenotype via HIF-dependent demethylation of SOX2 mRNA. Oncogenesis, 9(9), 81.
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3

Western Blot Analysis of Protein Expression

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Total protein was extracted and protein concentration was then measured by the DC protein assay method of Bradford (Bio-Rad, Hercules, CA, USA). Proteins were separated on sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (GE Healthcare, Piscataway, NJ, USA). Blots were immunostained with primary antibodies overnight and then with secondary antibody at room temperature for 1 h. Proteins of interest were visualized using ECL Plus Western blotting Detection Reagents (GE Healthcare). Antibodies against GAPDH (glyceraldehyde 3-phosphate dehydrogenase; sc-25778) and E-cadherin (sc-21791) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Cyclin D1 (no. 2978), p27 (no. 3686), Cleaved Caspase-7 (no. 9491) and Cleaved PARP (no. 5625) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against PCNA (ab29) was purchased from Abcam.
Yu J., Liang Q., Wang J., Wang K., Gao J., Zhang J., Zeng Y., Chiu P.W., Ng E.K, & Sung J.J. (2016). REC8 functions as a tumor suppressor and is epigenetically downregulated in gastric cancer, especially in EBV-positive subtype. Oncogene, 36(2), 182-193.
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