Ab155138

Manufactured by Abcam

Sourced in United Kingdom

Ab155138 is a laboratory equipment product. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

2 protocols using ab155138

Western Blot Analysis of VLCAD

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Cell lysates were diluted in Laemmli buffer and proteins were separated using a 12% polyacrylamide denaturating gel. Proteins were transferred to nitrocellulose membranes and incubated with 1:1000 anti-very long-chain acyl-CoA dehydrogenase (anti-VLCAD, ab155138, Abcam) or 1:500 anti-TOM20 (sc136211, Santa Cruz). Anti-ACTB (1:5000) (ab8226, Abcam) was used as a loading control. Fluorescent secondary antibodies (IRDye® anti-mouse and anti-Rabbit, 1:20000) were added to the membranes and bands were obtained using a near-infrared Odissey system. Bands were semi-quantified by densitometric analysis using ImageJ software.

Chausse B., Kakimoto P.A., Caldeira-da-Silva C.C., Chaves-Filho A.B., Yoshinaga M.Y., da Silva R.P., Miyamoto S, & Kowaltowski A.J. (2019). Distinct metabolic patterns during microglial remodeling by oleate and palmitate. Bioscience Reports, 39(4), BSR20190072.

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Protein Expression Analysis in Cells

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Equal amounts of proteins were separated using SDS-PAGE under reducing conditions. Proteins of interest were detected using the following antibodies; SIRT1 (S5196, Sigma-Aldrich), SIRT3 (5490S, Cell signaling), SIRT6 (GTX105611, Gene Tex), GLUT4 (sc53566, Santa Cruz Biotechnology), HK2 (2857, Cell signaling), PKM1/2 (3190, Cell signaling), PDHA1 (3205, Cell signaling), ACADVL (ab155138, Abcam), ACADL (ab82853, Abcam), CD36 (ABM-5525, Cascade Bioscience), AMPKα (G. Hardie, University of Dundee, Dundee, Scotland, UK), pAMPKα T172 (2531, Cell signaling), ACC (3661, Cell signaling), pACC S79 (3662, Cell signaling), PGC-1α (3242, EMD Millipore), ATP5A, UQCRC2, MTCO1, SDHB, NDUFB8 (MS604, MitoSciences), COX4 (4850P, Cell signaling), CYTC (11940, Cell signaling), eEF2 (2332, Cell signaling), Akt2 (2964, Cell signaling), pAkt S473 (9271, Cell signaling), pAkt T308 (9275, Cell signaling), GSK3α/β (5676, Cell signaling), pGSK3α/β S21/S9 (9331, Cell signaling). Densitometric analysis of immunoblots was performed on four or seven individual samples using Image Lab Software (Bio-Rad Laboratories), and a representative selection is presented in each figure.

Svensson K., LaBarge S.A., Martins V.F, & Schenk S. (2017). Temporal overexpression of SIRT1 in skeletal muscle of adult mice does not improve insulin sensitivity or markers of mitochondrial biogenesis. Acta physiologica (Oxford, England), 221(3), 193-203.

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