B220 perc

Manufactured by Thermo Fisher Scientific

The B220-PerC is a compact benchtop centrifuge designed for various laboratory applications. It features a maximum speed of 22,000 rpm and a maximum relative centrifugal force (RCF) of 48,400 x g. The centrifuge accommodates swing-out rotors that can hold microtubes, PCR strips, and various other sample containers. The B220-PerC is a versatile laboratory equipment piece.

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Lab products found in correlation

2 protocols using b220 perc

Multicolor Flow Cytometry of Splenocytes

Cited 1 time

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Single cell suspensions of splenocytes were treated with ACK lysis buffer (155 mM NH₄Cl, 10 mM KHCO₃, 100 μM EDTA) to remove red blood cells before staining in PBS, containing LIVE/DEAD fixable near-IR dead cell stain (Life Technologies) and appropriate, pre-titered antibodies. Antibodies used, indicating antigen and fluorophore (and clone): CD69-FITC (H1.2F3), CD86-PE (GL-1), Streptavidin-PerCP (Becton Dickinson), MHC class II-bio (I-Ab) (M5/114.15.2), IgM-PECy7 (II/41), B220-eFluor450 (RA3-6B2) (eBiosciences), CD19-FITC, B220-PerC, IgM-PE, IgG1-APC, PNA-FITC, CD38-PE/Cy7, CD45.2-ef450, CD45.1-FITC, CD138-PE, IgD-ef450, CD4-FITC, CD8-FITC, CD45.2-PerCP-cy5.5, CD45.1-FITC, B220-BV605, CD4-BV650, CD8a-BV650, CD38-bio, Streptavidin-PacO, FAS-PE. Anti-Syk Antibody (5F5) (Biolegend) and NIP-BSA were labelled using LYNX Rapid APC, RPE and PerCP Antibody Conjugation Kits (AbD Serotec). Antibody staining of intracellular Syk was carried out as previously described (13 (link)).

Ackermann J.A., Nys J., Schweighoffer E., McCleary S., Smithers N, & Tybulewicz V.L. (2015). Syk Tyrosine Kinase Is Critical for B Cell Antibody Responses and Memory B Cell Survival. The Journal of Immunology Author Choice, 194(10), 4650-4656.

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Single-Cell Flow Cytometry Analysis of Splenocytes

Cited 1 time

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Single-cell suspensions of splenocytes were treated with ACK lysis buffer to remove RBCs before staining in PBS, containing LIVE/DEAD fixable near-IR dead cell stain (Life Technologies) and appropriate, pretitered Abs. Abs used, indicating Ag and fluorophore (and clone): CD69-FITC (H1.2F3), CD86-PE (GL-1), streptavidin-PerCP (Becton Dickinson), MHC class II-bio (I-Ab; M5/114.15.2), IgM-PECy7 (II/41), B220-eFluor450 (RA3-6B2; eBioscience), CD19-FITC, B220-PerC, IgM-PE, IgG1-APC, PNA-FITC, CD38-PE/Cy7, CD45.2-ef450, CD45.1-FITC, CD138-PE, IgD-ef450, CD4-FITC, CD8-FITC, CD45.2-PerCP-cy5.5, CD45.1-FITC, B220-BV605, CD4-BV650, CD8a-BV650, CD38-bio, Streptavidin-PacO, FAS-PE. Anti-Syk Ab (5F5; BioLegend) and NIP-BSA were labeled using LYNX Rapid APC, RPE, and PerCP Ab Conjugation Kits (AbD Serotec). Ab staining of intracellular Syk was performed as described previously (13 (link)).

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