Stable isotope labeled adenosine 13c1015n5 triphosphate atp13c15n

Cytarabine for injection used in this study was purchased from Actavis Italy S.p.A. Human acute promyelocytic leukemia cell line HL-60 was supplied by American Type Culture Collection (ATCC) (Rockville, MD, USA). Phosphate buffer saline (PBS), RPMI medium 1640, 0.25% Trypsin-EDTA solution, penicillin-streptomycin solution, and fetal bovine serum (FBS) were obtained from GIBCO Invitrogen Co. (Carlsbad, CA, USA). A Cell Cycle Analysis Kit was purchased from Signal-way Antibody Co., Ltd. (College Park, MD, USA). Trichloroacetic acid (TCA), hexylamine (HA), diethylamine (DEA), trioctylamine, 1,1,2-trichlorotrifluoroethane and stable isotope labeled adenosine-¹³C₁₀¹⁵N₅-triphosphate (ATP¹³C¹⁵N) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). LC-MS grade methanol, acetonitrile, and acetic acid were purchased from Anaqua Chemical Supply Co. (Houston, TX, USA). Ultra-pure water was obtained from the Milli-Q Gradient Water System (Millipore Corporation, Billerica, MA, USA).

LC-MS grade methanol, acetonitrile and acetic acid were purchased from Anaqua Chemical Supply (Houston, TX, USA). Trioctylamine, 1, 1, 2-trichlorotrifluoroethane, methyl methanesulfonate (MMS), dimethyl sulfoxide (DMSO) and stable isotope labeled adenosine-¹³C10¹⁵, N5-triphosphate (ATP¹³C¹⁵,N) were purchased from Sigma Aldrich Chemical Co., St. Louis, MO, USA. Ultra-pure water was obtained from a Milli-Q Gradient water system (Millipore, Bedford, MA, USA). Dulbecco's Modified Eagle Medium (DMEM), RPMI Medium 1640, penicillin–streptomycin solution, fetal bovine serum (FBS) and SYBR^® safe DNA gel stain were obtained were obtained from Invitrogen Corp., CA, USA. Ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA-2Na) and Triton^® X-100 were bought from MP Biomedicals, LLC., Illkirch, France. Human hepatocellular cancer cell line (HepG2) was bought from American Type Culture Collection (ATCC; Rockville, MD, USA). Human hepatocyte normal cell line (LO2) was obtained from type Culture Collection of Chinese Academy of Sciences (Shanghai, China).