Multiple cryosections were obtained from HCT116 or HCT116 p53−/− tumor xenografts. The proliferative cells (Ki67+ area) were determined using a rabbit anti-Ki67 antibody (Novus Biologicals, Littleton, CO, USA) and peroxidase-conjugated goat anti-rabbit antibody (The Jackson Laboratory, Sacramento, CA, USA). Antibody binding was visualized using stable diaminobenzidine. Images were obtained in four different quadrants of each tumor section at ×40 magnification. Measurement of the area of Ki67-stained proliferative cells was done as described previously7 (link).
Rabbit anti ki67 antibody
Manufactured by Novus Biologicals
Sourced in United States
The Rabbit anti-Ki67 antibody is a laboratory tool designed for the detection and analysis of the Ki67 protein, a widely used marker of cell proliferation. This antibody specifically recognizes the Ki67 protein, which is expressed during all active phases of the cell cycle, but is absent in resting cells. The Rabbit anti-Ki67 antibody can be used in various immunoassay techniques, such as immunohistochemistry and flow cytometry, to quantify and visualize actively proliferating cells within a sample.
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Lab products found in correlation
2 protocols using rabbit anti ki67 antibody
1
Quantifying Tumor Cell Proliferation
2
Quantifying Proliferation in B16F10 Xenografts
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The proliferative cells were determined on the cryosections of B16F10 xenograft tumors using a rabbit anti-Ki67 antibody (Novus Biologicals, Littleton, CO, USA) and goat anti-rabbit antibody conjugated with peroxidase (The Jackson Laboratory, Sacramento, CA, USA). The proliferative cells (Ki67+ area) were visualized using stable diaminobenzidine. Images were obtained from each section at ×100 magnification. The area of Ki67-stained proliferative cells was examined as previously described [34 (link)].
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