Spheroids were imaged using an Olympus IX 81 optical microscope with a Hamamatsu digital camera and Slidebook image acquisition software (Slidebook 4.2.0.10, Olympus, Center Valley, PA, USA). ImageJ digital analysis software (https://imagej.net/ij , accessed on 22 January 2024) was used to measure the spheroid sizes on a minimum of 50 spheroids per condition.
Slidebook image acquisition software
Manufactured by Olympus
Sourced in United States
Slidebook is an image acquisition software designed for capturing, processing, and analyzing digital microscope images. It provides a user-friendly interface for controlling various imaging devices and managing the acquired data.
Automatically generated - may contain errors
2 protocols using slidebook image acquisition software
1
Spheroid Imaging and Measurement
2
Morphological Analysis of ADSCs
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The morphology of ADSCs isolated from well-vascularized and ischemic tissues were examined using an Olympus IX 81 optical microscope. Images were captured at three different locations per culture plate using a Hamamatsu digital camera connected to Slidebook image acquisition software (Slidebook 4.2.0.10, Olympus, Center Valley, PA, USA).
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