Commercially available lipases were used as follows: (i) Immobilized lipases:
Candida antarctica lipase type B immobilized on acrylic resin (CAL-B, 7,300.0 U/g), Thermomyces lanuginosus lipase immobilized on immobead-150 (TLL, 250.0 U/g), Rhizopus oryzae lipase immobilized on immobead-150 (ROL, 340.0 U/g), Pseudomonas fluorescens lipase immobilized on immobead-150 (PFL, ≥ 600 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel (AK, ≥ 30 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel on pumice (AK, ≥ 8.0 U/g) and Amano lipase PS from Burkholderia cepacia immobilized on diatomaceous earth (PS-IM, ≥ 500 U/g) were acquired from Sigma-Aldrich®, Rhizomucor miehei lipase immobilized in anionic resin (RML, 150.0 U/g) was purchased from Novozymes ® . (ii) Unsupported lipase preparations: Pseudomonas fluorescens lipase (AK, 22,100.0 U/g), Penicillium camemberti lipase (G, 50.0 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), Amano lipase from Mucor javanicus (MJL, ≥ 10,000 U/g) and lipase from Aspergillus niger (ANL, 200 U/g) were acquired from Sigma-Aldrich ® . Porcine pancreas lipase (PPL, 46.0 U/g solid), and Candida rugosa lipase (CRL, 1.4 U/g) were obtained from Sigma-Aldrich ® . (iii) Esterase, immobilized in Eupergit ® C from hog liver (~200 U/g) was obtained from Sigma-Aldrich ® .
Candida antarctica lipase type B immobilized on acrylic resin (CAL-B, 7,300.0 U/g), Thermomyces lanuginosus lipase immobilized on immobead-150 (TLL, 250.0 U/g), Rhizopus oryzae lipase immobilized on immobead-150 (ROL, 340.0 U/g), Pseudomonas fluorescens lipase immobilized on immobead-150 (PFL, ≥ 600 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel (AK, ≥ 30 U/g), Pseudomonas fluorescens lipase immobilized in Sol-Gel on pumice (AK, ≥ 8.0 U/g) and Amano lipase PS from Burkholderia cepacia immobilized on diatomaceous earth (PS-IM, ≥ 500 U/g) were acquired from Sigma-Aldrich®, Rhizomucor miehei lipase immobilized in anionic resin (RML, 150.0 U/g) was purchased from Novozymes ® . (ii) Unsupported lipase preparations: Pseudomonas fluorescens lipase (AK, 22,100.0 U/g), Penicillium camemberti lipase (G, 50.0 U/g), lipase from Rhizopus niveus (RNL, ≥ 1.5 U/mg), Amano lipase from Mucor javanicus (MJL, ≥ 10,000 U/g) and lipase from Aspergillus niger (ANL, 200 U/g) were acquired from Sigma-Aldrich ® . Porcine pancreas lipase (PPL, 46.0 U/g solid), and Candida rugosa lipase (CRL, 1.4 U/g) were obtained from Sigma-Aldrich ® . (iii) Esterase, immobilized in Eupergit ® C from hog liver (~200 U/g) was obtained from Sigma-Aldrich ® .