Amln diet

C57Bl/6J (wild-type, WT), Fbn1^NPS/+ mice (NPS), DIO, and leptin-receptor-deficient (Lepr^db/db) mice were purchased from Jackson Laboratories. Mice were fed normal chow (5V5R, Lab Supply), dustless pellet diet (F0173, Bio-Serv), high-fat diet (60% calories from fat, TD.06414, Envigo Teklad), or the Amylin diet rich in fructose and cholesterol in addition to fat (AMLN diet, D09100301, Research Diets), where indicated. Twelve-week-old C57Bl/6J WT male mice were used for adenovirus and adeno-associated virus studies and lean mice anti-asprosin mAb studies. Sixteen-week-old mice with DIO, maintained on high-fat diet for 12 weeks, were used for single-dose anti-asprosin mAb experiments, assessment of glucosuria, hepatic cAMP levels, mAb treatment-associated side effects, mAb half-life, and plasma markers of lipodystrophy assessment. Twenty-five- to thirty-week-old DIO mice were used for 14 days mAb study and MRI assessment. Thirty-week-old C57Bl/6J WT mice, maintained on 24 weeks of AMLN diet, and young (12-week-old) and old (30-week-old) Lepr^db/db mice were used for chronic mAb experiments. Thirty-five-week-old Fbn1^NPS/+ mice were used for the acute mAb experiment. All experimental mice were age and sex matched with control mice across all experiments in this study.