Luciferase based assay kit

Manufactured by Promega

Sourced in United States

The Luciferase-based assay kit is a laboratory tool used to measure the activity of luciferase, an enzyme that produces bioluminescence. The kit contains reagents and protocols to quantify luciferase levels in samples, facilitating research in areas such as gene expression, reporter assays, and cell-based studies.

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Lab products found in correlation

2 protocols using luciferase based assay kit

Mitochondrial Proteome Profiling and Function

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For extraction of mitochondrial and cytosolic proteins, a commercially available kit from Sigma-Aldrich (#MITOISO2, Saint Louis, MO, USA) was used. For staining of mitochondria, MitoProbe™ JC-1 (5,5ʹ,6,6ʹ-tetrachloro-1,1ʹ,3,3ʹ-tetraethyl-imidacarbocyanine iodide) (#M34152, Molecular Probes, Invitrogen, CA) was used. A luciferase-based assay kit was purchased from Promega Corp. (#G7570, Madison, WI). Parkin (#ab77924), TOM20 (#ab186735), TIM23 (#ab230253), PGC-1α (#ab191838), mt-TFA (#ab131607), LC3 (#ab51520), P62 (#ab56416), P62 phospho S349 (#ab211324), COX IV (#ab202554), NLRP3 (#ab214185), ASC (#ab175449), caspase-1 (#ab179515), cleaved caspase-3 (#ab49822), IL-1β (#ab9722), KIM-1 (#ab47635), Bax (#ab32503), Bcl-2 (#ab182858) and GAPDH (#ab181602) antibodies were purchased from Abcam (Cambridge, UK). The CellTiter-Glo assay and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) staining kit were obtained from Keygen Biotech (#KGA7037, Nanjing, China). ELISA kits to assay inflammatory cytokines (tumour necrosis factor-α [TNF-α, #1217202], interleukin-1β [IL-1β, #1210122], and interleukin-6 [IL-6, #1210602]) were obtained from Dakewe Biotech (Shenzhen, Guangdong, China). The immunohistochemical kits were obtained from Beyotime (#P0203, Shanghai, China), and all the other chemicals were purchased from Sigma-Aldrich (Saint Louis, MO, USA).

Gao Y., Dai X., Li Y., Li G., Lin X., Ai C., Cao Y., Li T, & Lin B. (2020). Role of Parkin-mediated mitophagy in the protective effect of polydatin in sepsis-induced acute kidney injury. Journal of Translational Medicine, 18, 114.

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Evaluating Cell Viability and ATP Levels

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RGC-5 cells (0.5 × 10⁴ per well) were plated on a 96-well plate. Cell viability was measured using 3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide (MTT) and a microplate reader (Spectra MAX; Molecular Devices Corp., US) [23 (link)]. The cellular ATP level was measured using a luciferase-based assay kit (Promega Corp., US) and a microplate luminometer (GMI Inc., US) [23 (link)].

Shim M.S., Kim K.Y., Noh M., Ko J.Y., Ahn S., An M.A., Iwata T., Perkins G.A., Weinreb R.N, & Ju W.K. (2018). Optineurin E50K triggers BDNF deficiency-mediated mitochondrial dysfunction in retinal photoreceptor cell line. Biochemical and biophysical research communications, 503(4), 2690-2697.

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