Eos 5d mark 2 dslr camera

Photographs were taken using a Canon EOS 5D Mark II DSLR camera with a Canon MP-E 65 mm 1–5× macro lens mounted on a motorized rail (Cognisys Stackshot). Elytral microreticulation was imaged using a Canon EOS 600D camera attached to a Leica Z6 APO macroscope with a 2× objective lens. Aedeagal apices of South African species were imaged with the same system, as temporary mounts in hand sanitizer gel. Images were stacked using the PMax algorithm in Zerene Stacker and manually edited in Photoshop. Boxplots were made using R version 3.4.3.

Liver lipids were extracted using a Folch technique as described previously [25 (link)]. Triglycerides and cholesterol were then measured using commercially available kits from Stanbio, as described previously [26 (link)]. Glycogen content was quantified using an assay kit from Abcam. For histological analysis, formalin-fixed livers embedded in paraffin wax were sectioned at 4 μm thickness and stained with Trichrome Stain Masson Kit (Sigma-Aldrich). All stained tissue sections were visualized by Olympus BX50 microscope and then photographed using a Canon EOS 5D Mark II DSLR camera at 10X magnification. Images were analyzed using Image Pro Plus 6.0 (Media Cybernetics).

Lipids were extracted using the Folch technique as described previously [38] (link). Triglycerides and cholesterol were then measured using commercially available kits as described previously [39] (link).
For histological analysis, formalin-fixed livers embedded in paraffin wax were sectioned at 4 µm thickness and stained with Trichrome Stain Masson Kit (Sigma-Aldrich). All stained tissue sections were visualized by Olympus BX50 microscope and then photographed using a Canon EOS 5D Mark II DSLR camera at 10X magnification. Images were analyzed using Image Pro Plus 6.0 (Media Cybernetics).