The apoptotic cell rates were determined using an Annexin-V-Phycoerythrin (PE) Apoptosis Detection Kit (Beyotime Biotechnology). Briefly, the cells were harvested and washed with PBS three times. Then cells were resuspended with 500 μl of 1× binding buffer and were stained with 5 μl of annexin V-PE for 15 min in the dark at 37°C. Cell apoptosis was measured using a FACScan (Beckman Coulter, Fullerton, CA, USA), and the data were analyzed utilizing FlowJo software (Tree Star Inc., Ashland, OR, USA).
Annexin 5 phycoerythrin pe apoptosis detection kit
Manufactured by Beyotime
Sourced in China
The Annexin-V-Phycoerythrin (PE) Apoptosis Detection Kit is a laboratory reagent used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule that is externalized during apoptosis. The Annexin V is conjugated to the fluorescent dye Phycoerythrin (PE) to enable detection and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
Facscan, by Beckman Coulter (1 mentions)
Foxo1 antibody, by Cell Signaling Technology (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Cell counting kit 8 cck 8 assay, by Dojindo Laboratories (1 mentions)
Terminal deoxynucleotidyl transferase dutp nick end labeling tunel kit, by Roche (1 mentions)
Anti β actin antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Horseradish peroxidase labeled secondary antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
2 protocols using annexin 5 phycoerythrin pe apoptosis detection kit
1
Annexin-V-PE Apoptosis Assay Protocol
2
Lentiviral Modulation of AQP9 in Hepatoma Cells
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Human hepatoma cell line SMMC7721 was purchased from the Cell Bank at the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. Lentivirus vector expressing AQP9-enhanced green fluorescent protein (eGFP) (named LV-AQP9) and lentivirus vector expressing eGFP (named LV-PWPI) were obtained from Shanghai Genechem Co., Ltd. (Shanghai, China). Annexin V-phycoerythrin (PE) apoptosis detection kit was purchased from Beyotime Institute of Biotechnology (Shanghai, China). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit was purchased from Roche (Basel, Switzerland). Cell counting kit-8 (CCK8) assay was purchased from Dojindo (Tokyo, Japan). Rabbit anti-human AQP9 antibody was purchased from Abcam (Cambridge, MA, USA). Rabbit anti-human caspase-3, PI3K, proliferating cell nuclear antigen (PCNA), Akt, P-Akt, and forkhead box protein O1 (FOXO1) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibody and horseradish peroxidase-labeled secondary antibodies were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China).
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