Peg4 spdp

Manufactured by Thermo Fisher Scientific

Sourced in United States

PEG4-SPDP is a heterobifunctional crosslinking reagent that contains a N-hydroxysuccinimide (NHS) ester and a pyridyldithio group. It is useful for conjugating proteins, peptides, and other biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

Lupasol g100, by BASF (1 mentions)

2 protocols using peg4 spdp

Aptamer-Crosslinked Hydrogel Bead Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

First, the prepared BSA-EDC hydrogel beads in PBS-EDTA buffer were filtered, and then 15 mg of beads were weighed into each needed well of a new 96-well plate. The beads were washed and resuspended 3 times in 250 µL PBS-EDTA buffer. In the next step, the supernatant was removed and 3.3 µL of PEG₄-SPDP (Thermo Fisher Scientific) (20 mM in DMSO) was pipetted onto the beads, and the well was filled up to 250 µL with PBS-EDTA and incubated while covered at 22 °C for 16 h. After that, the beads were washed 3 times with 250 µL of PBS-EDTA buffer. Next, the eight aptamers were mixed in the desired concentration and then activated to ensure the right folding. For this, the aptamer mix was heated up to 95 °C for 5 min and then cooled for 5 min on ice. After that, the aptamer solution was covered and stored at 22 °C for 30 min. Then, the aptamer solution was added to the well and filled up to 250 µL with PBS-EDTA buffer and incubated while covered for 1 h at 22 °C. After incubation, the aptamer-crosslinked BSA-EDC hydrogel beads were washed 3 times with 250 µL PBS-EDTA buffer.

Krämer M., Kissmann A.K., Raber H.F., Xing H., Favella P., Müller I., Spellerberg B., Weil T., Kubiczek D., Sihler S., Ziener U, & Rosenau F. (2021). BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing Pseudomonas aeruginosa in Serum and Blood. International Journal of Molecular Sciences, 22(20), 11118.

+ Open protocol

+ Expand

Synthesis and Characterization of Targeted Polyplexes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tf-PEI and Mel-PEI conjugates were prepared as previously described. [17 (link)] Briefly, Tf was coupled to 5 k PEI (Lupasol® G100, BASF, Ludwigshafen, Germany) via succinimidyl 3-(2-pyridyldithio) propionate (SPDP, Thermo Fisher Scientific, Waltham, USA) to yield the Tf-PEI conjugate with a reducible disulfide bond. Similarly, cysteine modified melittin (Mel, Pepmic, Suzhou, China) was coupled to PEI via 4-polyethylene glycol-N-succinimidyl 3-(2-pyridyldithio) propionate (PEG4-SPDP, Thermo Fisher Scientific, Waltham, USA) after masking of its lytic amine groups with 2,3-Dimethyl-maleic anhydride solution (DMMAn, Sigma-Aldrich, St. Louis, USA) to yield the pH-responsive Mel-PEI conjugate as described before.[21 ]
For Tf-Mel-PEI blend polyplexes, Tf-PEI and Mel-PEI were mixed in a 1:1 ratio and diluted in 5 % glucose. Defined amounts of siRNA were added to obtain specific amine to phosphate (N/P) ratios according to the following equation for calculating the mass of polymer required for 50 pmol siRNA: m (PEI in pg) = 50 pmol x 43.1 g/mol x N/P x 52 (protonable unit of PEI = 43.1 g/mol, number of nucleotides of 25/27mer siRNA = 52). Solutions were mixed by pipetting and incubated for 20 min at room temperature before proceeding with respective experiments.

Kandil R., Baldassi D., Böhlen S., Müller J.T., Keul D.C., Bargmann T., Dehmel S., Xie Y., Mehta A., Sewald K, & Merkel O.M. (2023). Targeted GATA3 knockdown in activated T Cells via pulmonary siRNA delivery as novel therapy for allergic asthma. Journal of controlled release : official journal of the Controlled Release Society, 354, 305-315.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!