Nusieve gtg agarose

Total RNAs were extracted in mice livers according to the manual specification using a TRI reagent RNA extraction kit (Fluka, Buchs, Switzerland). The concentration of total RNAs was measured by nano-drop spectrophotometry. RT-PCR analysis was performed for Tn-C and Fibronectin mRNA. Each sample was reverse-transcribed to cDNA for 1 hr at 42°C using a cDNA synthesis kit (Promega, Madison, WI, USA). PCR was done using the following primers: Tn-C ((Forward primer:5'-ATCGT-TACCGCCTCAACTACA-3'; Reverse primer: 5'-TGT-TCCATCCACAGTCACCA-3') [321 bp]); Fibronectin (sense sequence, 5-CAA TGC CCT TCC TGT TCT GC-3; anti-sense sequence, 5-GTG GAC GGC GTA GGC TTC TT-3 [452 bp]); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; sense sequence, 5-GTG GAT ATT GTT GCC ATC AAT GAC C -3; anti-sense sequence, 5-GCC CCA GCC TTC TTC ATG GTG GT -3 [270 bp]); all purchased from Bioneer (Daejeon, Korea).
The GAPDH was used to verify that equal amounts of RNA were used for reverse transcription and PCR amplification from different experimental conditions. Products were electrophoresed on a 2.0% NuSieve GTG-agarose (Cambrex Bio Science, Rockland, ME, USA) gel and visualized by staining with Safeview nucleic acid (Applied Biological Materials Inc, Vancouver, BC, Canada). The gels were certified using a Molecular Imager ChemiDoc XRS+ (Bio-Rad).